[Skip to Content]
[Skip to Content Landing]
Article
September 1989

Alternative to Flow Cytometry for the Study of Tumor Cell Kinetics

Arch Otolaryngol Head Neck Surg. 1989;115(9):1028. doi:10.1001/archotol.1989.01860330018006

This article is only available in the PDF format. Download the PDF to view the article, as well as its associated figures and tables.

Abstract

At the 31st annual meeting of the American Society for Head and Neck Surgery held in San Francisco, Calif, April 5 and 6, 1989, Marc Coltrera, MD, and colleagues of the University of Washington, Seattle, reported on a new alternative to flow cytometry for the study of cell kinetics within tumors. They noted that earlier studies have shown a correlation between the extent of cell proliferation within a tumor and the rate of local recurrence, distant metastases, and response to radiotherapy. Using a monoclonal antibody to PCNA (Proliferating Cell Nuclear Antigen) they compared immunoperoxidase staining of fixed, paraffin-embedded archival tumor tissue with previously obtained flow cytometric data on the same tissue. Proliferating cell nuclear antigen staining was recorded on a 1 to 4 scale depending on the proportion of cells staining the specimen. In general, a good correlation was found between the two methods. Discrepancies arose

First Page Preview View Large
First page PDF preview
First page PDF preview
×