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At the 31st annual meeting of the American Society for Head and Neck Surgery held in San Francisco, Calif, April 5 and 6, 1989, Marc Coltrera, MD, and colleagues of the University of Washington, Seattle, reported on a new alternative to flow cytometry for the study of cell kinetics within tumors. They noted that earlier studies have shown a correlation between the extent of cell proliferation within a tumor and the rate of local recurrence, distant metastases, and response to radiotherapy. Using a monoclonal antibody to PCNA (Proliferating Cell Nuclear Antigen) they compared immunoperoxidase staining of fixed, paraffin-embedded archival tumor tissue with previously obtained flow cytometric data on the same tissue. Proliferating cell nuclear antigen staining was recorded on a 1 to 4 scale depending on the proportion of cells staining the specimen. In general, a good correlation was found between the two methods. Discrepancies arose
WEISSLER MC, NC H. Alternative to Flow Cytometry for the Study of Tumor Cell Kinetics. Arch Otolaryngol Head Neck Surg. 1989;115(9):1028. doi:10.1001/archotol.1989.01860330018006