To improve the detection of p53 protein in formalin-fixed, paraffin-embedded head and neck tumor tissues.
University and Veterans Administration medical centers.
Patients or Other Participants:
Surgery for head and neck carcinoma.
Main Outcome Measure:
Retrieval of p53 antigen. Hypothesis formulated after data collection.
An antigen retrieval method facilitated the unmasking of previously inaccessible p53 antigenic determinants in formalin-fixed, paraffin-embedded tissues. This approach has made possible a much more reliable and sensitive immunohistochemical detection of p53 antigen. The procedure is simple, requiring only microwave heating of tissue sections to 100°C in the presence of a zinc sulfate solution.
Antigen retrieval method in formalinfixed, paraffin-embedded tissue demonstrated a significant increase in p53 immunostaining.(Arch Otolaryngol Head Neck Surg. 1993;119:1206-1209)
Pavelic ZP, Portugal LG, Gootee MJ, Stambrook PJ, Smith C, Mugge RE, Pavelic L, Wilson K, Buncher CR, Li Y, McDonald JS, Gluckman JL. Retrieval of p53 Protein in Paraffin-Embedded Head and Neck Tumor Tissues. Arch Otolaryngol Head Neck Surg. 1993;119(11):1206-1209. doi:10.1001/archotol.1993.01880230048008