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Correction
May 2005

Innate Immunity of the Sinonasal Cavity: Expression of Messenger RNA for Complement Cascade Components and Toll-like Receptors—Correction

Arch Otolaryngol Head Neck Surg. 2005;131(5):406. doi:10.1001/archotol.131.5.406

Errors in Text and Figures. In the Original Article by VanderMeer et al titled “Innate Immunity of the Sinonasal Cavity: Expression of Messenger RNA for Complement Cascade Components and Toll-like Receptors,” published in the December issue of the ARCHIVES (2004;130:1374-1380), there were errors in the text and figures. The first paragraph of the “Results” section should have appeared as follows: “Sha et al3 previously detected mRNA for all 10 known TLRs in human airway epithelial cells, leading us to expect that human sinonasal tissue samples may also contain mRNA for TLRs. Therefore, we tested tissue from human sinonasal mucosa for the presence of mRNA for TLR1-TLR10. Detectable levels of all 10 TLRs were found on screening the sinonasal tissue samples with standard reverse transcription PCR. To begin to assess the relative levels of TLRs, we developed TaqMan probes and primer sets for human TLRs (Table 1). Figure 1 shows the threshold cycle values for levels of mRNA for all 10 human TLRs quantified in 5 patients with sinusitis. Values were relatively consistent among the 5 subjects, and the SEM for each TLR was 0.67 or fewer PCR cycles. Among the TLRs, relatively high values were detected for TLR1, TLR2, TLR3, and TLR5; intermediate values were detected for TLR6, TLR7, TLR8, TLR9, and TLR10; and low values were detected for TLR4.”

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