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Correction
May 2005

Innate Immunity of the Sinonasal Cavity: Expression of Messenger RNA for Complement Cascade Components and Toll-like Receptors—Correction

Arch Otolaryngol Head Neck Surg. 2005;131(5):406. doi:10.1001/archotol.131.5.406

Errors in Text and Figures. In the Original Article by VanderMeer et al titled “Innate Immunity of the Sinonasal Cavity: Expression of Messenger RNA for Complement Cascade Components and Toll-like Receptors,” published in the December issue of the ARCHIVES (2004;130:1374-1380), there were errors in the text and figures. The first paragraph of the “Results” section should have appeared as follows: “Sha et al3 previously detected mRNA for all 10 known TLRs in human airway epithelial cells, leading us to expect that human sinonasal tissue samples may also contain mRNA for TLRs. Therefore, we tested tissue from human sinonasal mucosa for the presence of mRNA for TLR1-TLR10. Detectable levels of all 10 TLRs were found on screening the sinonasal tissue samples with standard reverse transcription PCR. To begin to assess the relative levels of TLRs, we developed TaqMan probes and primer sets for human TLRs (Table 1). Figure 1 shows the threshold cycle values for levels of mRNA for all 10 human TLRs quantified in 5 patients with sinusitis. Values were relatively consistent among the 5 subjects, and the SEM for each TLR was 0.67 or fewer PCR cycles. Among the TLRs, relatively high values were detected for TLR1, TLR2, TLR3, and TLR5; intermediate values were detected for TLR6, TLR7, TLR8, TLR9, and TLR10; and low values were detected for TLR4.”

In addition, Figure 1 and Figure 2 should have appeared as follows. The ARCHIVES regrets the errors.

Figure 1.
Real-time polymerase chain reaction (PCR) analysis of expression of toll-like receptors (TLRs) in human sinonasal tissue. Average cycle threshold (Ct) for messenger RNA (mRNA) of TLRs in human sinonasal tissue. In real-time PCR, fluorescence values are recorded during every PCR cycle and represent the amount of product amplified to that point in the amplification reaction. The more mRNA for a given gene that is present at the beginning of the reaction, the fewer number of cycles it will take for the fluorescent signal to reach a point that is statistically significant above background. The Ct is the cycle at which the fluorescent signal first increases above background. The total number of cycles performed in each experiment is 40. A Ct of 40 means that the fluorescence never reached above background level, therefore translating to an undetectable starting quantity of mRNA. The lower the Ct is below 40, the more mRNA is present in the sample, in an exponential fashion. Error bars represent SEM.

Real-time polymerase chain reaction (PCR) analysis of expression of toll-like receptors (TLRs) in human sinonasal tissue. Average cycle threshold (Ct) for messenger RNA (mRNA) of TLRs in human sinonasal tissue. In real-time PCR, fluorescence values are recorded during every PCR cycle and represent the amount of product amplified to that point in the amplification reaction. The more mRNA for a given gene that is present at the beginning of the reaction, the fewer number of cycles it will take for the fluorescent signal to reach a point that is statistically significant above background. The Ct is the cycle at which the fluorescent signal first increases above background. The total number of cycles performed in each experiment is 40. A Ct of 40 means that the fluorescence never reached above background level, therefore translating to an undetectable starting quantity of mRNA. The lower the Ct is below 40, the more mRNA is present in the sample, in an exponential fashion. Error bars represent SEM.

Figure 2.
Agarose gel electrophoresis of polymerase chain reaction amplification products encoding complement components in human liver (A) and human sinonasal tissue (B). Top columns: ladder; complement factors B, H, and I; and properdin. Bottom columns: ladder, mannose-binding lectin-associated serine protease 1 (MASP1), MASP2, mannose-binding lectin, C3, and β-actin. Representative of 9 separate samples (sinonasal) or a single sample (liver).

Agarose gel electrophoresis of polymerase chain reaction amplification products encoding complement components in human liver (A) and human sinonasal tissue (B). Top columns: ladder; complement factors B, H, and I; and properdin. Bottom columns: ladder, mannose-binding lectin-associated serine protease 1 (MASP1), MASP2, mannose-binding lectin, C3, and β-actin. Representative of 9 separate samples (sinonasal) or a single sample (liver).

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