It was first stated by Deschambs and Bussy (1852) that the reducing substance is usually found in the cerebrospinal fluid. Since that time numerous articles have appeared on this subject, and recent investigations have proved definitely that the reducing substance is glucose, and that it is always present in the spinal fluid in normal conditions.
At the present time, the methods most generally used in clinical laboratories for the determination of the glucose in the cerebrospinal fluid are those of Haines, Benedict and Lewis and Bang. The Haines method is employed simply for the qualitative determination of the glucose. For the quantitation of the glucose in the spinal fluid, the Benedict and Lewis method and the Bang micromethod give good results. Most American workers have employed the Benedict and Lewis method, or some modifications of it. Employing this method, Schloss and Schroeder1 found that the glucose content in normal
KASAHARA M, HATTORI S. A RAPID CLINICAL METHOD FOR THE DETERMINATION OF THE REDUCING SUBSTANCE IN THE CEREBROSPINAL FLUID: THE METHYLENE BLUE METHOD. Am J Dis Child. 1921;22(2):218-220. doi:10.1001/archpedi.1921.04120020115010