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While studying the effect of the immunization of children with diphtheria anatoxin in 1927, the author saw the need of a practical and reliable method for the determination of small amounts of diphtheria antitoxin in the blood. Ehrlich's classic method was not applicable to this work. The results with Romer's technic on guinea-pigs proved unsatisfactory. The intracutaneous procedure on rabbits proposed by Fraser and Wigham in 1924 was found to give the most favorable results, to be the most exact, rapid and least expensive and to be suitable for the determination of extremely small, as well as very large, amounts of toxin by a single technic. The author has further developed this procedure and intensively investigated all modifying factors and sources of error, and has perfected the method to a high point of accuracy. In this monograph these researches are described fully, and the directions for carrying out the tests
Die intrakutane Kaninchenmethode zur Auswertung von Diphtherie-Toxin und Antitoxin.. Am J Dis Child. 1933;46(2):465-466. doi:10.1001/archpedi.1933.01960020228022