[Skip to Content]
[Skip to Content Landing]
Other Articles
April 1941

A SIMPLE METHOD FOR THE ISOLATION AND IDENTIFICATION OF MENINGOCOCCI AND GONOCOCCI

Author Affiliations

NEW HAVEN, CONN.

Am J Dis Child. 1941;61(4):710-716. doi:10.1001/archpedi.1941.02000100044004
Abstract

An increased carbon dioxide tension has been employed for many years in the isolation of meningococci and gonococci. In 1918 Chapin1 described this method for the cultivation of gonococci, and in the same year Cohen and Fleming2 reported that there was a much better growth of meningococci when carbon dioxide was added in 10 per cent concentration. It is generally believed that these investigators probably thought that the improved growth of the organisms was due to the resultant decrease in oxygen tension, since this was the prevailing opinion at that time. The value of the method has been corroborated on numerous occasions, although there have been differences of opinion as to the factor primarily responsible. In addition to the decrease in oxygen tension, an increase in moisture and the production of a more favorable ph have been considered. In 1924 Smith3 found that carbon dioxide was

First Page Preview View Large
First page PDF preview
First page PDF preview
×