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May 1942

METHOD OF ASSAYING TRYPSIN SUITABLE FOR ROUTINE USE IN DIAGNOSIS OF CONGENITAL PANCREATIC DEFICIENCY

Author Affiliations

NEW YORK
From the Babies Hospital and the Departments of Pediatrics and Pathology, Columbia University College of Physicians and Surgeons.

Am J Dis Child. 1942;63(5):891-893. doi:10.1001/archpedi.1942.02010050055005
Abstract

The diagnosis of congenital pancreatic deficiency can be established by the determination of the concentration of pancreatic enzymes in the duodenal juice in suspected cases.1 Of all the diagnostic methods explored, the assay of trypsin has been found to be the most valuable procedure. The method used for the determination of trypsin in the previous study was a viscosimetric one chosen for reliability and sensitivity. This method has the disadvantage of requiring a fairly elaborate equipment, much of which is not found in a hospital routine laboratory. Since the difference between the level of trypsin in congenital pancreatic deficiency and that in other varieties of the celiac syndrome is a wide one, a less sensitive and less elaborate method should be adequate for diagnosis and more practical for routine use. Several methods were tried, and the method of Fermi,2 modified by Palitzsch and Walbum3 and again by

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