The existence of a large number of serologically distinct types of Escherichia coli and the pathogenic significance of a number of these strains in the causation of both epidemic and sporadic diarrheas of infants are well recognized. The rapid detection and identification of enteropathogenic E. coli strains has become a necessity for epidemiologic and clinical control of infantile diarrheal disease. Conventional bacteriologic and serologic methods usually require at least 24 to 48 hours. The fluorescent antibody technique, by which a large number of different micro-organisms and viruses have already been identified,1-11 seemed especially applicable to the problem of pathogenic E. coli. This paper describes the application of the fluorescent antibody method as a diagnostic test for identification of E. coli O127:B8, which can be carried out within 15 to 25 minutes directly from stool specimens or rectal swabs.
Materials and Methods
An epidemic of diarrhea in 1954 in
WHITAKER J, PAGE RH, STULBERG CS, ZUELZER WW. Rapid Identification of Enteropathogenic Escherichia Coli O127:B8 by the Fluorescent Antibody Technique. AMA Am J Dis Child. 1958;95(1):1-8. doi:10.1001/archpedi.1958.02060050003001