Sickling can be produced in the blood of young Negro babies with sickle-cell trait. The percentage of cells showing this change is low but will increase as the infant matures. The homozygous state is present from birth; however, recognition of the symptomatic condition is rare in infants under the age of 4 months. Investigation of the literature reveals only four case reports in infants less than 3 months of age. These cases predate the period when electrophoretic and biochemical procedures could be applied. This article concerns case reports of two infants with sickle-cell anemia who were studied with these methods.
The erythrocyte and leukocyte counts were made by routine methods. Hemoglobin was determined as cyanmethemoglobin with a Klett-Summerson photoelectric colorimeter.1 The hematocrit values were determined by the Wintrobe method. Tests for sickling were performed by the sealed cover-slip method, with use of sodium metabisulfite as a reducing agent.
LEIKIN SL, McCOO JW. Sickle-Cell Anemia in Infancy. AMA Am J Dis Child. 1958;96(1):51-57. doi:10.1001/archpedi.1958.02060060053009