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March 1962

Infectivity Stability of Live Measles-Virus Vaccine

Author Affiliations

H. Goldner, Ph.D., Division of Virus and Tissue Culture Research, Merck Institute for Therapeutic Research, West Point, Pa.; From the Division of Virus & Tissue Culture Research, Merck Institute for Therapeutic Research.; Research Associate (Dr. Goldner, Dr. Buynak) and Director (Dr. Hilleman), Division of Virus & Tissue Culture Research, Merck Institute for Therapeutic Research.

Am J Dis Child. 1962;103(3):440-442. doi:10.1001/archpedi.1962.02080020452060

Thermal instability and infectivity loss on drying are well-recognized properties of measles virus.1-4 Obviously, these difficulties must be overcome in preparing live measles-virus vaccine for general use.

The present brief report summarizes early findings in investigations of stability of liquid, frozen, or dried measles-virus vaccine prepared in our laboratories.

Materials and Methods  Vaccine.—All lots of Enders' live attenuated measles-virus vaccine (Edmonston strain) were prepared in tissue cultures of chick embryo according to Enders et al.4 Dried vaccine contained an added stabilizer. Frozen vaccine was stored at —70 C in a solidified carbon dioxide (Dry Ice) refrigerator in flame-sealed ampoules. Dried vaccine was sealed in vacuo in ampoules with rubber closures. These were capped with aluminum seals and covered with a thermoplastic wax to provide a moisture barrier. Rehydration was carried out with sterile distilled water without preservative. Pools of 3 vials were made for titration purpose.Infectivity

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