February 1970

Acute Parotitis Associated With Parainfluenza 3 Virus Infection

Author Affiliations

From the Division of Pediatrics and the Department of Virology, Cook County Hospital and the Hektoen Institute for Medical Research (Drs. Zollar and Mufson); the Department of Pediatrics, Chicago Medical School (Dr. Zollar); and the Department of Medicine, University of Illinois College of Medicine (Dr. Mufson), Chicago.

Am J Dis Child. 1970;119(2):147-148. doi:10.1001/archpedi.1970.02100050149012

Mumps virus represents the single most important etiological agent of acute epidemic parotitis. However, the isolation of coxsackieviruses type A, echoviruses, choriomeningitis virus, and parainfluenza 1 virus from a few individuals with a clinically similar illness, who often relate a prior episode of mumps, suggests that other viruses may cause acute parotitis, albeit infrequently.1,2 In this report, we describe the recovery of parainfluenza 3 virus and detection of significant rises in homologous hemagglutination-inhibition antibody in two children with acute parotitis, one of whom previously had mumps.

Materials and Methods  Oropharyngeal swab specimens collected in chilled sterile veal infusion broth containing 0.5% bovine serum albumin were inoculated fresh into roller tube cultures of rhesus monkey kidney, HEp-2, and human fetal diploid fibroblast (WI-38) cells. The maintenance media and handling of cell cultures has been reported in detail.3Microtiter assay of hemagglutination-inhibition antibody titers for parain-fluenza viruses types 1, 2,

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