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Article
July 1971

CREATINE PHOSPHOKINASE ACTIVITY IN CEREBROSPINAL FLUID-Reply

Author Affiliations

Torrance, Calif

Am J Dis Child. 1971;122(1):86-87. doi:10.1001/archpedi.1971.02110010121027

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Abstract

Meltzer is quite correct with regard to the expression of creatine phosphokinase activity as international units per liter of milliunits per milliliter. One international unit of CPK will catalyze the transfer of 1 μmol of phosphate between creatine phosphate and ADP. The temperature control was maintained to the utmost possibility. Creatine phosphokinase standards were utilized in the preliminary phases of this study to insure the validity of the method. In addition, replicate analyses were performed on all serum and CSF specimens to insure the accuracy of reported values. The dilution modification of two parts CSF to one part of a specific premixed ingredient (Supercalsul) was used to aid in the detection of the low CSF CPK activity. Furthermore, the Supercalsul method is specific for creatine phosphokinase activity. Thus, the reliability of the reported values was believed to be quite good. Therefore, Katz and I referred to

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