June 1972

Identification of Translocation Chromosomes by Quinacrine Fluorescence

Author Affiliations

Southbury and New Haven, Conn; New York
From Southbury Training School, Southbury, Conn, and the Division of Medical Genetics, Department of Pediatrics, Yale University School of Medicine, New Haven, Conn (Dr. Breg); and the Department of Obstetrics and Gynecology and of Human Genetics and Development, Columbia University College of Physicians and Surgeons, New York (Drs. Miller, Allderdice, and Miller).

Am J Dis Child. 1972;123(6):561-564. doi:10.1001/archpedi.1972.02110120085007

Quinacrine dihydrochloride fluorescence patterns permit the identification of every normal human metaphase chromosome. To determine the effectiveness of the quinacrine fluorescence method in detecting and delineating reciprocal translocation chromsomes, individuals with such abnormal chromosomes were studied. The fluorescence patterns provided a means for the rapid identification of translocation chromosomes, many of which were extremely difficult or impossible to detect and identify by previous methods. Six patients had different translocations: (1) t(2p−;11p + ), (2) t(3p−; 18q + ), (3) translocation in which the arms of a No. 2 and a No. 6 had interchanged, (4) t(5p−;14q + ), (5) one in which arms of a No. 11 and a No. 17 were interchanged, and (6) t(1q−;18p + ). Studies by methods which do not permit such exact identification of all the chromosomes are no longer adequate in clinical cytogenetics.