A, Errors for the expectancy AX task. For trial type, the immediate and delay conditions were combined. B, Distributions of patients, siblings, and control subjects according to BX−AY differences. Data are given as mean (SE). AX indicates a trial type in which A precedes X; AY, a trial type in which A precedes Y (any non-A probe); BX, a trial type in which B (any non-A probe) precedes X; and BY, a trial type in which B precedes Y.
Interference on correct trials for the expectancy AX task. For interference type, the immediate and delay conditions were combined. Data are given as mean (SE). AX indicates a trial type in which A precedes X; AY, a trial type in which A precedes Y (any non-A probe); BX, a trial type in which B (any non-A probe) precedes X; and RT, reaction time.
MacDonald AW, Pogue-Geile MF, Johnson MK, Carter CS. A Specific Deficit in Context Processing in the Unaffected Siblings of Patients With Schizophrenia. Arch Gen Psychiatry. 2003;60(1):57-65. doi:10.1001/archpsyc.60.1.57
Understanding the biological basis of complex, heritable illnesses such as schizophrenia is facilitated by sensitive and functionally specific measures of intermediate processes. Context processing is a theoretically motivated construct associated with executive function. Impairments in this process have been associated with dysfunction of the prefrontal cortex. In the present study, we evaluated whether a specific deficit in context processing could be associated with the unexpressed genetic liability to schizophrenia.
Twenty-four patients with schizophrenia, 24 unaffected siblings and 36 control subjects completed a version of the AX task with (1) a condition that required context processing and (2) an expectancy condition in which intact context processing could lead to errors.
Patients and unaffected siblings performed relatively worse in the context processing condition, whereas controls performed relatively worse in the expectancy condition. A double dissociation between siblings and controls (F = 9.5, P<.005) constituted strong evidence of a specific deficit in context processing associated with a familial or genetic liability to schizophrenia. Preliminary evidence of high diagnostic efficiency was also noted (specificity, 38%; and sensitivity, 100%).
Context processing deficits have been associated with dorsolateral prefrontal cortex dysfunctions in schizophrenia. Such a dysfunction may occur even when genetic liability to schizophrenia is unexpressed clinically. The present method of demonstrating a double dissociation may be a useful approach to exploring endophenotypes related to specific cognitive and neural processes that can be measured in ways sensitive to subtle group differences.
NEW APPROACHES to determining the nosology and etiology of schizophrenia have focused on disentangling the complex features of the illness into basic heritable processes that may lead to the symptoms of schizophrenia.1 Such traits are often easier to study and can be more closely linked to underlying biological processes, such as neural activity and genetic expression. However, to our knowledge, no studies have demonstrated endophenotypes that are specific impairments in healthy individuals who are likely to have a genetic liability to schizophrenia. The present study was conducted to determine whether context processing, a theoretical construct characterized by Cohen and Servan-Schreiber,2 is a specific deficit in the healthy relatives of patients with schizophrenia. Such a finding could be a useful model for the study of other traits associated with complex genetic illnesses.
In the pursuit of genes related to the etiology of schizophrenia, there has been much emphasis on identifying endophenotypes, which are measures sensitive to the unexpressed genetic liability to schizophrenia.3 One approach has been to use batteries of standard neuropsychological tests to determine cognitive domains associated with the largest effect sizes.4- 6 A second approach has been to optimize the difficulty of tasks sensitive to deficits in patients with schizophrenia.3,7- 13 Both approaches have demonstrated impairments on various attentional control and memory tasks. However, an impairment on a given task is not the same as an impairment on a given cognitive function, ie, these impairments have not yet been shown to be specific, or differential, deficits.14 The alternative explanation of poor task performance is that patients and their relatives have generalized deficits and that attentional control and memory tasks tend to have more discriminating power than other tasks.
The generalized deficit confound is important to address in family studies of schizophrenia. First, the confound is a source of noise for molecular genetic studies that use endophenotypes. The purpose of an endophenotype is to increase statistical power to detect liability genes by identifying gene carriers who do not manifest clinical symptoms.1,15 However, this potential gain in power may be offset if the endophenotype is merely sensitive to heterogeneous causes of generalized deficits (which include lower intelligence, differences in motivation and other personality factors, or deficits in other cognitive processes). Specific deficits should be easier than generalized deficits to relate to neural structures, neurotransmitter systems, or genetic polymorphisms. Second, identifying a specific deficit may allow task manipulations to amplify the signal associated with a particular cognitive process, thereby increasing our ability to detect the unexpressed genetic liability to schizophrenia in future studies. Finally, specific impairments may be useful targets for potential preventative treatments.
The most popular solution to the generalized deficit confound has been to match tasks of interest with control tests on properties such as difficulty, observed variance, and reliability, thereby equating the tasks to be compared for discriminating power.16 Two studies17,18 that have used such matching strategies in the unaffected adult relatives of patients with schizophrenia have not yielded significant effects. Another approach is to isolate specific deficits by including within the experiment internal controls that constrain the interpretation of the impaired cognitive process.19 The most powerful internal control is one that results in a double dissociation. In this case, psychometric properties are not matched across condition. Instead, the experimental group performs worse because of the task demands in one condition, while the control group performs worse on a second condition. The present study used such an approach to study the theoretically motivated construct of context processing.
Context processing refers to the adaptive control of current behavior through the use of prior context information. Context information might include the processing of a sequence of stimuli, such as a sentence; a specific prior stimulus, such as a cue; and a set of task instructions. Context processing is conceptualized as consisting of 2 intertwined components, representation and maintenance. Representation refers to the process of constructing and using the context information to control behavior. Maintenance refers to the short-term storage of the context representation to control behavior even after a delay. Thus, context processing can be classified as an executive function that subsumes aspects of other cognitive constructs, including response inhibition, selective attention,20 cognitive control,21 and working memory.22 Previous research on context processing in the general population has demonstrated that this construct can be modeled as top-down support for task-specific processes using a simple connectionist architecture,2 and that representation and maintenance of information are associated with dorsolateral prefrontal cortex function.23 Patients with schizophrenia show context processing deficits24- 26 that correlate with the prominence of disorganization symptoms.27 These deficits seem to be related to a circumscribed dorsolateral prefrontal cortex dysfunction in long-term–medicated28 and medication-naive patients,29 and can be modeled as a dopamine dysfunction that interferes with top-down support.30,31
In developing a task sensitive to subtle, but specific, context processing deficits in the unaffected relatives of patients, we modified the expectancy AX (trial type) task used in recent studies24,27 of patients with schizophrenia. In this task, an X is only a target when it follows an A. We strengthened the automatic, or default, pattern of responding by making most trials AX pairs, thus establishing that As were generally followed by targets and Xs were generally valid targets. Three exception trial types were included: AY, BX, and BY pairs (where B was any non-A cue and Y was any non-X probe). This expectancy manipulation tested participants' ability to use the context of the cue to overcome their automatic target response to the probe: AY false alarms increased if the A cue was strongly represented because Y violated the expectation that A is generally followed by a valid probe (X); and BX false alarms increased if the B cue was weakly represented because, although nothing following a B could be a target, X was generally a valid probe. False alarms in all conditions, including the AX and BY conditions, increased in the presence of a generalized deficit. The interval between the cue and the probe was also varied from no delay to a short delay. This allowed us to partially disentangle the 2 components of context processing, representation and maintenance. Both conditions required context representation, but maintenance was required more in the delay condition.
The present study evaluated whether context processing deficits are characteristic of the unexpressed liability to schizophrenia. Specifically, we tested the hypothesis that patients with schizophrenia and their unaffected siblings would show more errors and slower reaction times (RTs) in the BX relative to the AY condition. We hypothesized that this effect would increase when the cue was followed by a delay. Furthermore, we hypothesized that the expectancy AX task would be diagnostically efficient, in the sense that it would be useful in identifying individuals more likely to express schizophrenia-related genes.5
Twenty-four medicated patients with schizophrenia or schizoaffective disorder, 1 unaffected sibling of each patient, and 36 control subjects participated. The demographic characteristics of the final sample are summarized in Table 1. Thirty-one outpatients at Western Psychiatric Institute and Clinic, Pittsburgh, volunteered to participate based on a DSM-IV medical record diagnosis of schizophrenia or schizoaffective disorder that was verified using the Structured Clinical Interview for DSM-III-R.32 For each patient, we attempted to recruit 1 full sibling between the ages of 18 and 45 years who had not previously been diagnosed as having a psychotic disorder. Only patients with a participating full sibling were included in the analysis; of all potential patient families, 4 could not be reached, 1 refused, and 2 could not confirm full sibling status. There were no demographic, symptom, or functioning differences between patients who were included and those who were not. Controls were recruited through newspaper advertisements. Controls were invited to participate if they were between the ages of 18 and 45 years, had no history of psychosis, and were similar to the group of patient siblings in parental educational level. The reading section of the Wide Range Achievement Test III33 was given to all subjects as a measure of general intellectual ability, and the results are also reported in Table 1. There were no significant differences across the groups for any demographic variables, although patients showed a strong trend toward less education relative to controls.
Siblings and controls were screened for lifetime psychiatric symptoms or disorders using the Structured Clinical Interview for DSM-III-R: Non-patient Edition34 and the Structured Interview for DSM-III Personality Disorders–Revised,35 diagnosed as having any features according to DSM-IV, and rated on current psychiatric symptoms according to the Brief Psychiatric Rating Scale36 and the Positive and Negative Syndrome Scale.37 Interviews were conducted by one of us (A.W.M.) and a research assistant, both of whom participated in regular drift prevention training throughout the study. A total of 10 controls and siblings (17% of the sample) provided reliability for diagnostic ratings, and 13 additional patients (5 of whom participated in the study, or 21% of the sample) were included for reliability on symptom measures. There was good agreement across raters (κ = 0.74 for agreement across all diagnostic categories) and strong reliability for symptom ratings (Positive and Negative Syndrome Scale disorganization items intraclass correlation coefficient [ICC], 0.84; positive symptom items ICC, 0.87; negative symptom items ICC, 0.75; Brief Psychiatric Rating Scale total ICC, 0.94; and Global Assessment Scale ICC, 0.91). There were no significant (P>.10 for all) differences between siblings and controls for history of mood disorders (3 [12%] of 24 vs 2 [6%] of 36), anxiety disorders (4 [17%] of 24 vs 2 [6%] of 36), substance use disorders(4 [17%] of 24 vs 4 [11%] of 36), or any Axis I disorder (8 [33%] of 24 vs 7 [19%] of 36). Cluster A personality disorders were only observed in siblings (paranoid personality disorder, 2 siblings [8%] [P =.09]; schizoid personality disorder, 1 sibling [4%] [P =.21]; schizotypal personality disorder, 1 sibling [4%] [P = .34]; and any cluster A personality disorder, 3 siblings [12%][P = .06]). Although this was not significantly different from controls, secondary analyses were conducted without these subjects. As reported in Table 1, there were no significant differences between siblings and controls for ratings of current psychiatric symptoms, whereas patients showed significant impairment across all symptoms.
The AX-CPT was modified from a previous version27 and extensively repiloted in an initial sample. The task was performed on a personal computer (Macintosh; Apple Computer Inc), with white letters on a dark background appearing one at a time. Subjects identified targets and nontargets with a button press using separate fingers on the same hand according to the conditional A then X rule previously described. Subjects were instructed to respond as quickly and accurately as possible and were told that everyone makes some mistakes because speed is important. The experimenter remained in the testing room and repeated the instruction again during the session. In both counterbalanced conditions, the cue (A or non-A [hereafter B]) duration was 1000 milliseconds, and the probe (X or non-X [hereafter Y]) duration was 500 milliseconds. In the immediate condition, there was 0 millisecond between the cue and the probe and 7000 milliseconds before the next trial, whereas in the delay condition there were 6000 milliseconds between the cue and the probe and 1000 milliseconds before the next trial. Accuracy and millisecond RT data were available for targets and nontargets, and auditory feedback was provided for each response. The immediate and delay conditions each consisted of 4 blocks of 38 letter pairs, of which 30 (79%) were AX trials, 3 (8%) were AY trials, 3 (8%) were BX trials, and 2 (5%) were BY trials (which were included as a general manipulation check).
Analyses for all variables were performed first in controls to determine the nature of normative performance. For the between-group analysis, a 4 × 2 × 3 mixed-effects repeated-measures analysis of variance was conducted on the error data, with trial type (AX, AY, BX, or BY) and delay condition (immediate or delay) as within-subject factors and group (patient, sibling, or control subject) as the between-subject factor. Greenhouse-Geisser (G-G) corrections were used to control for assumption violations as necessary. Contrast analyses were conducted to examine group main effects and interactions between group and the trial types of interest to the context-processing hypothesis (AY vs BX). While the comparison of AY with BX trials controls for generalized deficit confounds that may be reflected as a response bias, a variant of d′, referred to as d′-context,24 was also examined. d′-Context is conceptualized as the proportion of trials in which participants use context to distinguish target signals relative to the proportion of trials in which they fail to use context to distinguish nontarget signals, or more formally, d′-context = z[AX hits] − z[BX false alarms], where z indicates the point on the axis of a normal curve corresponding to the proportion of AX hits and BX false alarms, respectively. In the case of a perfect hit or false-alarm score, a small constant was substituted to allow estimation.38 Reaction times for correct trials only were also analyzed as a measure of processing speed when task demands were met. Correct RTs between 150 milliseconds and 1500 milliseconds were subject to a 4 × 2 × 3 mixed-effects repeated-measures analysis of variance similar to that described for error data. To adjust for the large baseline RT differences frequently found between control and patient groups while allowing for an evaluation of the added processing requirements of AY and BX trials, a secondary measure of RT was calculated and analyzed. For this measure, called RT interference, the mean trimmed RT of correct AX trials was subtracted from the means of trimmed RTs for correct AY and BX trials.(BY was not used as a baseline because there were too few trials.) Univariate effect sizes were calculated per the method of Cohen.39
Discriminant functions were calculated to evaluate how effectively these measures separated siblings from controls. Finally, we conducted an exploratory diagnostic efficiency analysis to determine what cut points would provide adequate sensitivity and specificity for molecular genetic analyses.3
The expectancy AX task yielded error and RT data for 4 different trial types in 2 different delay conditions across 3 different groups. Table 2 summarizes error and d′-context data, and Table 3 summarizes correct trial RT and RT interference data across the different trial types, delays, and groups, along with the univariate effect sizes. We present controls data first, followed by between-group contrasts. Finally, we examine whether this instrument can efficiently identify siblings and controls.
Analyses of the error data, correct trial RT data, and secondary measures were initially performed in controls to evaluate the normative pattern of performance. For the error data, there was a main effect of trial type (G-G F1.36,105 = 28.37, P<.001), due to increased errors on AY trials, that was predicted. The nonsignificant effect of delay (F1,35 = 3.31, P = .08) and the significant interaction of trial type and delay (G-G F1.70,105 =3.71, P<.05) suggested that the immediate condition was overall easier than the delay condition because of better performance in the AX and BX conditions. A priori contrasts of AY and BX trials alone also showed the predicted interaction (F1,35 = 21.32, P<.001), with more errors on AY trials, as well as an interaction between trial type and delay (F1,35 = 4.81, P = .35), with increased BX errors with delay. Similarly, d′-context was significantly better in the immediate condition (F1,35 = 30.69, P<.001).
Correct trial RT analyses recapitulated the pattern observed in the error data. Again, there was the predicted main effect of trial type (G-G F2.27,105 = 230.11, P<.001), with longer RTs on correct AY trials and shorter RTs on BX trials. The effect of delay was highly significant (F1,35 = 101.24, P =.001), with the immediate condition faster than the delay condition, whereas the interaction between trial type and delay was not significant (P = .41). A priori contrasts of the AY and BX conditions showed a strong effect (F1,35 = 278.28, P<.001), with AY trials being slower. As with errors, this important effect did not interact with delay. As in the comparison of correct RTs, RT interference in controls showed a main effect of trial type (F1,35 = 278.27, P<.001), with AY trials slower than AX trials (positive interference) and BX trials slightly faster than AX trials. This effect did not interact with delay.
Thus, a normative pattern of performance is characterized by a high level of AY errors and slower RTs in both delay conditions and a moderate, but significant, increase in AX and BX errors with delay. These data are consistent with the interpretations that AY errors and slower RTs result from normal intact context processing.
Error data, correct trial RT data, and secondary measures were subsequently included in between-group analyses. Error analyses are reported in Table 2 and illustrated in Figure 1. As predicted, there was a significant group effect and a group–by–trial type interaction. A priori contrasts of overall AY and BX errors across groups showed the predicted differences between patients and controls (F1,81 = 14.70, P<.001) and between patients and siblings (F1,81 = 8.50, P<.01), but no overall performance difference between siblings and controls (F1,81 = 0.41, P = .52). The interaction of AY and BX trials with group was significant when comparing patients with controls (F1,81 = 10.73, P<.005) and siblings with controls (F1,81 = 9.58, P<.005), but there was no interaction of trial type and group in patients and siblings(F1,81 = .027, P = .87). These effects were due to increased BX errors in parallel for patients and siblings and increased AY errors in controls relative to siblings (Figure 1A). This predicted pattern of contrast effects provides strong interpretive leverage for describing the nature of context-processing impairments. The lack of an overall error effect between siblings and controls and the highly significant disordinal (x-shaped) interaction with trial type is suggestive of a double dissociation. In fact, controls made significantly more AY errors (t57.62 = −2.03, P<.05), while siblings made significantly more BX errors(t25.48 = 2.16, P<.05)(both 2-tailed tests for unequal variances). The difference between subjects' BX and AY errors was a useful metric that capitalized on this double dissociation, and subtracted out generalized deficits that were measured in both conditions. As expected, there were large mean differences on BX−AY between controls and both patients and siblings (effect sizes, 0.88 and 0.80, respectively), but patients were nearly equal to siblings (effect size, 0.05) (Figure 1B). The 3-way interaction in the omnibus tests was not interpretable(Table 2).
An analysis of the secondary measure, d′-context, is also reported in Table 2.
Again, a group effect was observed such that patients were less sensitive than controls or siblings (F1,81>12.00, P<.005, for both) and siblings tended to be less sensitive than controls (F1,81 = 3.46, P = .06).
Correct RT analyses are reported in Table 3. There were the predicted group differences and a group–by–trial type interaction that largely recapitulated the error effects: contrasts showed that patients were generally slower than siblings (F1,80 = 18.71, P<.001), siblings were generally slower than controls(F1,80 = 13.08, P<.005), and patients and siblings were significantly slower than controls on BX relative to AY trials (F1,80 = 19.24 and 4.99, respectively; P<.05 for both). There were also large effect size differences between groups on all but AY trials.
Reaction time interference data are particularly important for comparing groups with different baseline RTs and are also reported in Table 3 and illustrated in Figure 2. Most important, there was again the predicted main effect of group, which again interacted with trial type: contrasts showed that patients had more overall interference than either siblings or controls (F1,80 = 4.88 and 8.95, respectively; P<.05 for both), whereas siblings and controls did not differ; however, patients and siblings showed more BX interference, whereas controls showed more AY interference(F1,80 = 19.24 and 4.99, respectively; P<.05 for both), while patients and siblings did not show differences in interference between trial types.
The same pattern of effects was observed when 3 siblings with Axis II diagnoses were removed (eg, the group– by–trial type interaction for errors was G-G F3.97,234 = 4.51 [P<.005] and the contrast of siblings and controls on AY vs BX trials was F1,78 = 9.54 [P<.005]). In another set of subsequent analyses, other diagnoses were entered as covariates into a 4 × 2 × 3 analysis of covariance and the effects remained similar (eg, for all group–by–trial type interactions for errors, F>4.4 [P<.005]; and for the contrast of siblings and controls on AY and BX trials, F>7.9 [P<.005]). Thus, the pattern of results seemed closely tied to the genetic liability to schizophrenia and not to the sporadic occurrence of psychiatric symptoms in the healthy samples.
To summarize, across several distinct measures of impairment, patients showed a generalized deficit (poorer performance across all trials) in addition to a context processing impairment (poorer performance on BX trials relative to other trial types, especially AY trials). Siblings of patients showed a context processing impairment but not a generalized deficit. In contrast to patients and their siblings, controls performed worst on AY trials, presumably because they were being misled by their expectation of the probe. This suggests intact context processing. The context processing impairments in patients and their siblings occurred when the cue was immediately followed by the probe, as well as when there was a delay.
Exploratory discriminant functions were used to determine which measures or combinations of measures were more effective in separating the control and sibling groups. The BX−AY errors and BX − AY RTs were also examined to capitalize on the disordinal interaction while preserving df values. The most effective measure when entered alone was the mean of BX errors (68.3% correctly classified; sensitivity, 41.7%; specificity, 86.1%; Wilks Λ = 0.89; P<.05) and the RT for delay BX trials minus delay AY trials (66.1% correctly classified; sensitivity, 47.8%; specificity, 77.8%; Wilks Λ = 0.91; P<.05). Other BX−AY difference scores and RTs significantly, but less effectively, classified cases (P<.05), whereas d′-context did not significantly (P =.11) classify cases. Using multiple variables to separate groups did not significantly improve these classifications.
Diagnostic efficiency analyses were conducted to identify optimal cut points maximizing correctly classified siblings, or sensitivity, relative to correctly rejected controls, or specificity. A cut point of mean BX−AY errors greater than 0.20 showed 21% sensitivity at 100% specificity (χ21 = 8.18, Fisher exact test P<.01). A cut point of mean BX − AY RT greater than −32 showed 33% sensitivity at 100% specificity (χ2 = 13.85, Fisher exact test P<.001). A combination of these criteria led to 38% sensitivity at 100% specificity (χ2 = 15.88, Fisher exact test P<.001). These are post hoc analyses and, therefore, require replication. Nevertheless, they demonstrate that this version of the expectancy AX task, used as either a continuous or a dichotomous measure, may be a sensitive and specific probe of an endophenotype related to the genetic liability to schizophrenia.
Patients with schizophrenia and their unaffected siblings were compared with controls on an expectancy version of the AX task to test the hypothesis that context processing is a specific deficit associated with genetic liability. Siblings and patients performed better on AY relative to BX trials, which implied worse context processing, whereas controls performed better on BX relative to AY trials, which implied better context processing. The pattern of disordinal interactions between siblings and controls constituted strong evidence of a specific deficit in context processing associated with a familial, and probably a genetic, liability to schizophrenia. The observation that there was no main effect for performance indicates that our interpretation was not confounded by factors such as a general performance deficit, recruitment bias, speed-accuracy trade-off differences, or differences in vigilance. Any of these may have led to an increase in errors and/or RT interference across conditions rather than the pattern of strengths and deficits observed in siblings. This pattern was observed even after removing individuals with schizophrenia-spectrum disorders and variance associated with other Axis I psychopathological features.
Several studies have demonstrated impaired performance on continuous performance test paradigms in the relatives of patients with schizophrenia. Continuous performance tests that combine several sources of difficulty by including encoding, updating, and storage task demands have led to a consistent robust effect size of around 0.90.11- 13 This success has pointed the way, but has not yet established a specific deficit associated with genetic liability to schizophrenia. Two other studies have attempted to establish specific deficits in the relatives of patients with schizophrenia using tasks that required processing information in the face of distraction. One study18 found no difference between adult relatives when comparing a shorter digit span task with distractors with a longer digit span task without distractors that was psychometrically matched (a specific deficit was reported in high-risk adolescents using a similar task40). In another study,17 patients' relatives were not differentially impaired on a task that required repeating aloud audibly presented phrases in the presence of distractors compared with a matched task that only required repeating unpredictable words. The present study suggests that task manipulations that lead to better and worse performance in different task conditions may be a powerful strategy for establishing specific deficits, particularly when comparing 2 psychiatrically healthy groups. Thus, the comparison of AY hits with BX false alarms was more sensitive than the comparison of AX hits with BX false alarms (the components of d′-context). In this regard, the finding that RT differences on AX and BY trials showed as large between-group effects as did RTs on BX trials remains somewhat curious. On the one hand, this suggests that generalized deficits, as measured by correct RTs, can obscure the relative size of the specific context processing impairment. On the other hand, differential impairments on BX trials remained even after AX differences were subtracted away in the interference analysis. This suggests that RT difference scores may be a useful way to observe interpretable specific deficits.
One hypothesis that was not supported was that patients and their siblings would be more impaired after a delay. Consistent with some previous work,26 the present findings suggest that context processing deficits occur immediately and are not only due to the context representation degrading more rapidly in patients and siblings over the interval examined in the present paradigm. One might speculate that a longer delay would have resulted in significant effects.
The present study was also unable to address questions about the variation observed within the sibling group. Supplementary mixture analyses did not provide strong evidence either for or against a commingling of distinct subgroups. These questions remain important issues to address in future family studies.
Functional neuroimaging studies suggest that representing and maintaining a cue to overcome an automatic response, as in the current task, involves the dorsolateral prefrontal cortex23,41 and that this region is dysfunctional in patients with schizophrenia.28,29,42,43 Preliminary data from one study44 that used functional magnetic resonance imaging and a working memory task that required storage and executive processes showed that the unaffected siblings of patients were also impaired in activating this region of the cortex. Thus, there is early suggestive evidence that the specific deficits associated with the liability to schizophrenia may involve abnormal gene expression related to the function of the dorsolateral prefrontal cortex. Methods such as those illustrated herein could be used to test this hypothesis directly.
In conclusion, the present study evaluated context processing, a specific theoretically derived cognitive function, in the unaffected relatives of patients with schizophrenia. The modification of a classic task proved to be sensitive to subtle group differences, while at the same time retaining specificity to processes that have previously been localized to a particular region of the brain. It seemed that context processing was robustly related to the genetic liability to schizophrenia. Such translational cognitive neuroscience research may play an important role in the development of sophisticated tools for investigating the complex genetics of schizophrenia.
Corresponding author and reprints: Angus W. MacDonald III, PhD, Department of Psychology, University of Minnesota, Elliot Hall, 75 E River Rd, Minneapolis, MN 55455 (e-mail: email@example.com).
Submitted for publication November 8, 2001; final revision received May 20, 2002; accepted June 5, 2002.
This study was supported by a grant from the National Alliance for Research on Schizophrenia and Depression, Great Neck, NY; and grant MH64190 from the National Institute of Mental Health, Rockville, Md (Dr Carter).
This study was presented (as preliminary data) at the annual meeting of the Behavior Genetics Association, Burlington, Vt, June 30, 2000; and the biennial meeting of the International Congress on Schizophrenia Research, Whistler, British Columbia, May 2, 2001.
We thank the study participants and Judy Thompson, MS, Laura Ross, BA, Christy Belanger, BA, Eric Yablonsky, BA, and other students who assisted in this project.
This study constituted a portion of the doctoral thesis of Dr MacDonald for the Department of Psychology, University of Pittsburgh.