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May 1928

AN EXPERIMENT WITH BROTH CULTURES OF STAPHYLOCOCCUS AUREUS AND GENTIAN VIOLET

Author Affiliations

WASHINGTON, D. C.
From the Louis Bowles Foundation of the Children's Hospital School, Baltimore.

Arch Surg. 1928;16(5):1078-1079. doi:10.1001/archsurg.1928.01140050102007
Abstract

Following the lines of experimentation of Churchman1 in 1922, when he showed that a slight increase in temperature had a definite effect on the bacteriostatic power of gentian violet, and of Gatch, Trusler, and Owen2 in 1925, when they showed that gentian violet in 4 per cent dextrose was less toxic for rabbits than aqueous gentian violet, experiments were performed in test tubes and on agar plates to correlate these facts.

Twenty-four hour broth cultures of Staphylococcus aureus containing about 9 cc. of fluid were used. Gentian violet was prepared first with distilled water as a medium and then with 4 per cent dextrose as a medium. Sufficient amounts of the gentian violet preparations were added to the broth cultures to make a 1: 10,000 dilution of the dye. A bath at 50 C. was used to give the increase in temperature. A series of six broth cultures

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