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DETERMINATION of the sulfobromophthalein appearance-time in bile is not a laboratory procedure but a simple clinical method. Its measurement is made by the combination of duodenal intubation with the usual intravenous injection of sulfobromophthalein (150 mg. per square meter). For this purpose, the biliary fluid is simply collected by siphonage into tubes containing a few drops of 10% sodium hydroxide solution; these tubes are shaken every minute until a reddish-violet color develops. In order that the test be reliable (a) the output of biliary juice must be sufficient (about 1 cc. per minute as an average), and (b) it must contain a detectable amount of bile. Moreover, the biliary flow can be stimulated by intraduodenal instillation of procaine hydrochloride or magnesium sulfate.
In normal subjects the sulfobromophthalein appearance time ranges between 5 and 15 minutes. This test is of significant value in uncommon cases of subtotal and yet latent common
Caroli J. SULFOBROMOPHTHALEIN APPEARANCE-TIME IN BILEIts Diagnostic Value in Incomplete Obstructive Jaundice and in Nonicteric Obstruction of the Common Bile Duct. AMA Arch Surg. 1953;66(4):397-398. doi:10.1001/archsurg.1953.01260030412001