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December 1957

Technique for Collection of Sterile Bile from Experimental Animals

Author Affiliations

New Orleans
From the Department of Surgery, Louisiana State University School of Medicine.

AMA Arch Surg. 1957;75(6):1003-1005. doi:10.1001/archsurg.1957.01280180135020

Difficulty in obtaining large quantities of sterile bile has led to the development of several techniques in various animal species for prolonged collection of bile. The continuing sterility of these methods is open to serious question.

In the rat, which has no gallbladder, bile has been collected by cannulating the common duct and allowing bile to drain into specially constructed glass bulbs which were placed either in the peritoneal cavity1,2 or on the abdominal wall.3 Bile was withdrawn at specified intervals from the glass bulbs through rubber tubes. Another technique used a long plastic tube extending from the common duct through a subcutaneous tunnel to the dorsal surface of the rat's foot to permit continuous collection of bile while the animal was moving about.4

In the rabbit the common duct has been divided between ligatures, and a flexible tube inserted into the gallbladder and brought to the

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