Two methods can be used for perfusing small animals. Either the heart and lung of a second animal can be used as pump and oxygenator or a small mechanical oxygenator can be used.
With a mechanical pump oxygenator, the liver, lung, and spleen can be excluded in the perfusion of an extremity, for example. The exclusion of these organs is particularly helpful for a study of the perfusion of colloids, since these organs can remove some colloids in a single passage of blood.1
For a research program on chemically modified antibodies for cancer therapy, antibody complexes with a variety of radioactive and nonradioactive colloids are being investigated. The cytotoxic colloids being used include the fissionable actinides and boron-10 for the neutron capture therapy of tumors2; β and γ emittors; magnetic particles such as iron oxide for inductive heating of tumors, and suitably insolubilized antimetabolites and chelating agents. In
KNOCK FE. Disposable Pump Oxygenator for Perfusion in Small AnimalsUsed in Perfusing Chemically Modified Antibodies. Arch Surg. 1960;81(4):668-670. doi:10.1001/archsurg.1960.01300040152029