It has been demonstrated in this laboratory1-6 that acid-pepsin digested bovine tracheal cartilage accelerates the healing of wounds when applied locally in the form of microparticles or when administered subcutaneously by cartilage pellet implantation. The latter effect is dependent upon the time of pellet implantation relative to the making of the test incision, reaching a maximal positive effect with an interval of 3 to 5 days prior to the test incision. This documentation of the effect of a parenteral cartilage preparation on an anatomically distant wound is significant because it seemingly predicts that it is possible to isolate a water-soluble material from cartilage which is well tolerated parenterally and which, by virtue of its high potency, will permit a marked and controlled clinical acceleration of wound healing. This paper reports the initial progress which has been made toward the attainment of this goal.
Two basic techniques have been
PRUDDEN JF, GABRIEL O, ALLEN B. The Acceleration of Wound HealingUse of Parenteral Injections of a Saline Cartilage Extract, with a Note on the Evaluation of Electrophoretically Separated Fractions of the Extract by Tissue Culture. Arch Surg. 1963;86(1):157-161. doi:10.1001/archsurg.1963.01310070159020