This experiment was undertaken to develop a method of in vitro preservation of tissue in a viable state. Skin, bone, dura mater, and blood vessels have been transplanted successfully after periods of in vitro storage, but these tissues are usually preserved in a nonviable state and have been selected more for their structural or physical properties than for their physiological function. Tissue culture techniques have, of course, been highly developed, and strains of normal and neoplastic cells have been maintained for long periods of time. Certain aspects of tissue culture work have been applied to this problem in an effort to preserve thyroid tissue in vitro at low temperature and to demonstrate its viability upon reimplantation in the donor animal.
Thyroid tissue was selected because viability could be demonstrated by its uptake of radioactive iodine as well as by histological study. Endocrine tissue is well suited for transplantation because it
ZUIDEMA GD, BURTON WD. Transplantation of Autologous Canine ThyroidTransplantation After Preservation at Low Temperature. Arch Surg. 1963;86(2):246-249. doi:10.1001/archsurg.1963.01310080070016