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April 1966

Tumor CytotoxicityQuantitative Changes in DNA Synthesis as an Indicator

Author Affiliations

From the departments of surgery and biochemistry, University of Illinois College of Medicine, and Presbyterian-St. Luke's Hospital, Chicago.

Arch Surg. 1966;92(4):541-547. doi:10.1001/archsurg.1966.01320220097016

A MAJOR problem remaining in cancer chemotherapy is the difficulty in determining which drug will produce the maximal cytotoxic effect in the tumor of an individual patient. Previous studies in this laboratory described a method of evaluating cytotoxicity by observations of changes in deoxyribonucleic acid (DNA) synthesis.1 The present study examines the validity of using quantitative changes in DNA synthesis as an indicator of cytotoxicity in a population of tumor cells exposed in vivo to antitumor drugs.

The in vivo effects of two groups of drugs on DNA synthesis were evaluated in the Ehrlich ascites carcinoma maintained in CF-1 mice. One group of drugs had been shown to be carcinostatic for the Ehrlich ascites tumor at the indicated dosage levels as determined by total packed cell volumes in previous tumor screening procedures.2 The second group of drugs had been shown not to be carcinostatic at the indicated dosage

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