Implants of Walker 256 carcinosarcoma were placed between the thyroid isthmus and trachea of rats. After the tumor had invaded the trachea, the tumor mass and the involved area of trachea were frozen with a cryoprobe. Necropsy studies demonstrated complete erradication of tumor without permanently altering the gross architecture of the trachea. Cryotherapy may be of value in the management of selected patients with neoplasms of the trachea.
Skivolocki WP, Pace WG, Thomford NR. Effect of Cryotherapy on Tracheal Tumors in Rats. Arch Surg. 1971;103(2):341-343. doi:10.1001/archsurg.1971.01350080257041