September 1971

The Binding of Aldosterone to Echerichia coli Endotoxin

Author Affiliations

From the departments of surgery and biological chemistry, Hahnemann Medical College, Philadelphia.

Arch Surg. 1971;103(3):402-405. doi:10.1001/archsurg.1971.01350090084018

Aldosterone has been reported to lower the fatality rate in laboratory animals administered a lethal dose of endotoxin. It has been suggested that this beneficial effect of aldosterone may be due to a physical combination of the steroid with endotoxin, which would result in a decreased toxicity of the endotoxin. To test this hypothesis, the binding capacity of Escherichia coli endotoxin for aldosterone was measured by Sephadex filtration chromatography, and by comparing the rate of dialysis of aldosterone in the presence and absence of endotoxin. Within the limitations of both of the methods used, the results of this investigation indicate that E coli endotoxin does not have a strong affinity for d-aldosterone in purified systems.