March 1972

A Technique for Long-Term Intravenous Feeding in Unrestrained Rats

Author Affiliations

From the Harrison Department of Surgical Research (Dr. Vars) and the Department of Surgery (Drs. Steiger and Dudrick), University of Pennsylvania School of Medicine, and the Philadelphia Veterans Administration Hospital, Philadelphia.

Arch Surg. 1972;104(3):330-332. doi:10.1001/archsurg.1972.04180030076017

Equipment, techniques, and solutions have been developed for the chronic intravenous feeding of rats. With use of a special harness and fluid infusion swivel assembly, 140- to 250-gm, unrestrained rats can be fed for weeks at a time exclusively by vein, allowing for prolonged periods of metabolic and nutritional study. A 32% dextrose and 5% amino acid solution containing essential minerals and vitamins was formulated for the intravenous feeding regimen. This model can be readily utilized and has great potential value for studying problems related to surgical nutrition and metabolism.