June 1972

The Use of Tolonium in the Diagnosis of Malignant Gastric Lesions

Author Affiliations

Los Angeles
From the Division of Surgery and the Department of Experimental Surgery, Medical Research Institute, Cedars-Sinai Medical Center, Cedars of Lebanon Hospital, Los Angeles.

Arch Surg. 1972;104(6):773-777. doi:10.1001/archsurg.1972.04180060023005

The oral and intravenous administration of tolonium chloride (toluidine blue), a metachromatic dye, has been studied in 15 rats with alcohol-induced benign gastric ulcers and in 55 rats with malignant gastric lesions induced by 3-methylcholanthrene. Results in the series treated intravenously were unsatisfactory. Oral administration of 2 ml of 1.0% tolonium chloride gave the most effective results. Eight of 28 benign lesions were stained, but these occurred in rats killed within 12 hours of dye administration. Twelve of 13 malignant lesions were stained, with four of the lesions occurring in rats killed more than 32 hours after administration of the dye. The data suggest that oral administration of tolonium may aid in the endoscopic and surgical identification of malignant gastric lesions.