August 1972

The Crossmatch Profile and Other Extended Crossmatch Methods in Clinical Transplantation

Author Affiliations

Philadelphia; Albany, NY
From the research and surgery services, Albany Veterans Administration Hospital; the Surgery Service, Albany Medical Center Hospital; and the Department of Surgery, Albany Medical College of Union University, Albany, NY. Dr. Colberg is now with Jefferson Medical College, Philadelphia.

Arch Surg. 1972;105(2):237-241. doi:10.1001/archsurg.1972.04180080091015

Preexisting humoral immunity to donor alloantigens was investigated in 67 potential renal transplant patients on chronic hemodialysis on a continuing basis over 28 months by 2,740 cytotoxicity tests. The cytotoxicity tests were enhanced by the use of six concentrations of recipient sera, and other techniques for better assessment of immunity. The six-well crossmatch concentration profile demonstrated 596 discordant results (21.8%) from those obtained by a single-well crossmatch. Among these were 196 positive profiles which were negative in the customary 1λ well. The profile method further demonstrated a low number of false positives (32) and resolved 100 of 116 doubtful reactions. Dilutions appeared more valuable than concentrations in determining the final crossmatch. No cases of hyperacute or accelerated rejection occurred in 20 cadaver and 9 related kidney transplants.