Twenty-four canine small bowels were used to compare the different perfusion and nonperfusion systems previously studied in kidney preservation. Four systems were evaluated: (a) two pulsatile perfusion systems (Mox-100 and Mox-300), (b) a nonperfusion technique (Collins method), and (c) a combined method (Collins method for 6 hours followed by pulsatile perfusion for 18 hours). Final evaluation was determined by restoration of function in long-term (> 20 days) small bowel transplant survivors. All the dogs receiving an allotransplant of bowels continuously perfused were long-term survivors. Causes of death were related to cachexia, obstruction, or rejection. The animals receiving nonperfused bowels developed hemorrhagic necrosis and died 2.8 ± 1.2 days after transplantation.
Toledo-Pereyra LH, Najarian JS. Small Bowel PreservationComparison of Perfusion and Nonperfusion Systems. Arch Surg. 1973;107(6):875-877. doi:10.1001/archsurg.1973.01350240045013