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August 1980

Endothelial Cell Seeding of Prosthetic Vascular GraftsEarly Experimental Studies With Cultured Autologous Canine Endothelium

Author Affiliations

From the Departments of Anatomy and Surgery, University of Michigan Medical School, Ann Arbor.

Arch Surg. 1980;115(8):929-933. doi:10.1001/archsurg.1980.01380080025005

• Thirteen adult dogs underwent thoracoabdominal bypass operations with 6-mm, double-velour Dacron grafts 25 to 30 cm long. Experimental grafts were seeded with cultured autologous endothelial cells (n = 7). Unseeded grafts served as controls (n = 6). Endothelial cells were harvested from external jugular vein segments using 0.1% trypsin and 0.5% collagenase solutions. Grafts were studied at weeks 2 and 4. Endothelial cell coverage of experimental graft surfaces after two weeks was 60% to 70%, and approximately 80% after four weeks. Immunofluorescence using factor VIII-related antigen confirmed the graft's inner surface to be endothelium. Endothelial cell coverage in control grafts occurred as pannus ingrowth, and never exceeded more than 10% of the conduit surface. Generation of an early endothelial surface in prosthetic grafts is possible in a canine model using cultured autologous cells.

(Arch Surg 115:929-933, 1980)