[Skip to Content]
Access to paid content on this site is currently suspended due to excessive activity being detected from your IP address 54.166.61.50. Please contact the publisher to request reinstatement.
[Skip to Content Landing]
Article
January 1984

Inhibition of Bacterial Clearance in the Guinea Pig by Fluid-Phase C3b

Author Affiliations

From the Departments of Surgery (Dr C. Ogle) and Biological Chemistry (Dr J. Ogle). University of Cincinnati Medical Center; and the Shriners Burn Institute, Cincinnati (Drs Alexander and J. Ogle).

Arch Surg. 1984;119(1):57-60. doi:10.1001/archsurg.1984.01390130043008
Abstract

• The large-molecular-weight activation product C3b of C3, the third component of complement, loses its opsonic properties when it is free in solution (fluid phase) rather than attached to a particle, eg, a bacterial cell. Human fluid-phase C3b, which has been shown in vitro to be an inhibitor of the bactericidal function of human neutrophils, was injected intradermally or intraperitoneally into guinea pigs along with various species of bacteria, and the clearance rate of the bacteria was measured. The injected C3b caused a large increase in the time required for the clearance of the bacteria. The inhibition by C3b of clearance of the injected bacteria was dependent on the bacterial species; bacteria that depended strongly on complement for opsonophagocytosis was cleared at a much lower rate in the presence of fluid-phase C3b than were bacteria that did not have the corresponding dependence on complement.

(Arch Surg 1984;119:57-60)

×