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February 1990

Metastatic Potential of Colon CarcinomaExpression of ABO/Lewis-Related Antigens

Author Affiliations

From the Departments of General Surgery (Drs Hoff and Ota), Tumor Biology (Drs Irimura and Matsushita), and Pathology (Dr Cleary), University of Texas M. D. Anderson Cancer Center, Houston, and the Biomembrane Institute and University of Washington, Seattle (Dr Hakomori).

Arch Surg. 1990;125(2):206-209. doi:10.1001/archsurg.1990.01410140084013

• Four monoclonal antibodies specific to various Lewisx-related antigens were tested for binding to monolayers and detergent extracts of the human colon carcinoma cell line HT-29 P and its metastatic variant HT-29 LMM. Only monoclonal antibody FH6 (antisialyl-dimeric Lex) bound more to the metastatic variant compared with HT-29 P. Detergent extracts of human tissues were then assayed for their FH6-binding activity. Normal colonic mucosa displayed less FH6 binding than either Dukes stage B or D primary colon carcinomas. Liver metastases from colorectal carcinoma demonstrated greater binding than stage B and stage D primary tumors, and more than normal liver. Expression of sialyl-dimeric Lex increases as the metastatic potential of colorectal carcinomas increases, and is related to the progression of colorectal cancer.

(Arch Surg. 1990;125:206-209)