December 1990

Neuropeptide Y Inhibition of Vasoactive Intestinal Polypeptide–Stimulated Ion Transport in the Rabbit Distal Colon

Author Affiliations

From St Mary's Hospital, Waterbury, Conn (Drs Flint and Fielding), the Gastrointestinal Surgery Research Unit, Department of Surgery, Yale University School of Medicine, New Haven, Conn (Drs Ballantyne, Goldenring, and Modlin); and West Haven (Conn) Veterans Affairs Medical Center (Drs Ballantyne, Goldenring, and Modlin).

Arch Surg. 1990;125(12):1561-1563. doi:10.1001/archsurg.1990.01410240039008

• We have studied the effect of neuropeptide Y on basal and vasoactive intestinal polypeptide–stimulated changes in the short-circuit current of strips of colonic mucosa from New Zealand white rabbits mounted in Ussing chambers. When administered to the basolateral surface, neuropeptide Y is found to decrease basal short-circuit current. Neuropeptide Y inhibits vasoactive intestinal peptide–stimulated increases in short-circuit current in a concentration-dependent fashion by a tetrodotoxin-insensitive mechanism. Also, neuropeptide Y inhibited increases in short-circuit current produced by direct stimulation of adenylate cyclase with forskolin. Furthermore, neuropeptide Y prevents vasoactive intestinal peptide–stimulated increases in tissue cyclic adenosine monophosphate levels. These results indicate that neuropeptide Y administered to the basolateral membrane inhibits vasoactive intestinal peptide–stimulated short-circuit current changes by a tetrodotoxin-insensitive mechanism that decreases tissue levels of cyclic adenosine 3′,5′-monophosphate.

(Arch Surg. 1990;125:1561 -1563)