February 1991

Effect of Dietary Fish Oil on Plasma Thromboxane B2 and 6-Keto-prostaglandin F1α Levels in Septic Rats

Author Affiliations

From the Department of Surgery, the University of North Carolina at Chapel Hill. Dr Muakkassa is now with the University of Mississippi Medical Center, Jackson, and Dr Ramadan is now with the University of Pittsburgh (Pa).

Arch Surg. 1991;126(2):179-182. doi:10.1001/archsurg.1991.01410260067009

• Increased mortality from sepsis is associated with high levels of thromboxane B2 (TXB2) and 6-keto-prostaglandin F (PGF). Linoleic acid, an n-6 essential fatty acid, is the usual precursor of TXB2 and PGF, while fish oil is rich in n-3 essential fatty acid, the precursor of less active moieties. Rats were fed chow, an essential fatty acid–deficient diet, or an essential fatty acid–deficient diet supplemented with linoleic acid or fish oil for 2 weeks. The animals then underwent a sham operation or cecal ligation and puncture to induce sepsis. Six hours later, blood was obtained for analysis. The chow and linoleic acid diets produced significant (twofold to fivefold) increases in levels of both TXB2 and PGF after sepsis. The essential fatty acid–deficient diet and fish oil diet protected against increases in levels of TXB2 or PGF during sepsis. Dietary restriction of linoleic acid or fish oil supplementation may play an important role in altering the inflammatory mediator response to sepsis.

(Arch Surg. 1991;126:179-182)