May 1993

Determination of Tumor Aggressiveness in Colorectal Cancer by K-ras-2 Analysis

Author Affiliations

From the Department of Pathology, Rhode Island Hospital, Brown University, Providence.

Arch Surg. 1993;128(5):526-532. doi:10.1001/archsurg.1993.01420170056008

• Markers that predict tumor aggressiveness on a case-by-case basis would enable individualization and optimization of oncologic therapy. To achieve this goal, the presence and specific type of K-ras-2 point mutation was determined from formalin-fixed, paraffin-embedded tissue sites in 247 primary and 166 metastatic-recurrent colorectal adenocarcinomas, using a novel approach consisting of topographic tissue selection, DNA amplification, and direct sequencing applicable to large and needle-biopsy-sized specimens. The results provide the basis for a genotypic classification of colorectal cancer capable of predicting individual tumor aggressiveness, including the pattern and extent of metastasis.

(Arch Surg. 1993;128:526-532)