[Skip to Content]
Access to paid content on this site is currently suspended due to excessive activity being detected from your IP address 54.145.232.99. Please contact the publisher to request reinstatement.
[Skip to Content Landing]
Article
June 1995

Chromosome 17p Allelic Loss in Colorectal CarcinomaClinical Significance

Author Affiliations

From the Departments of Surgery (Drs Takanishi and Michelassi), Pathology (Drs Yaremko and Montag), and Medicine (Dr Westbrook), The University of Chicago (III); and the Clinica Chirurgica I, Universita' di Padova (Italy) (Dr Angriman)

Arch Surg. 1995;130(6):585-589. doi:10.1001/archsurg.1995.01430060023005
Abstract

Objective:  To correlate allelic losses on chromosomes 5q, 8p, 17p, and 18q in colorectal adenocarcinomas with histopathologic features of known prognostic significance.

Design:  DNA was extracted from paired samples of 56 fresh-frozen colorectal adenocarcinomas (one classified as Dukes' stage A, 22 as Dukes' stage B, 27 as Dukes' stage C, and six as Dukes' stage D) and adjacent normal mucosa.

Setting:  Specimens were resected at the University of Chicago (III) and the University of Padova (Italy) in 1991.

Patients:  Samples were obtained from consecutive patients.

Interventions:  Chromosomes 5q, 8p, 17p, and 18q were studied for loss of heterozygosity by means of Southern hybridization blot analysis of restriction fragment length polymorphisms, and the results were correlated with pathologic tumor stage, degree of differentiation, and lymphatic and/or vascular microinvasion.

Results:  Chromosomes 17p and 18q exhibited the highest frequency of loss of heterozygosity (40.6% and 48.8%, respectively) Most of the allelic losses were found in advanced tumors (60% in Dukes' stages C and D combined). A statistically significant correlation was found between loss of heterozygosity on chromosome 17p and the presence of lymphatic and/or vascular microinvasion (P<.01, Fisher's Exact Test).

Conclusions:  There was a significant correlation between loss of heterozygosity on chromosome 17p and the presence of lymphatic and/or vascular microinvasion in colorectal adenocarcinoma, a known stage-independent negative prognostic risk factor. Detection of loss of heterozygosity on chromosome 17p may identify a group of patients who may benefit from more aggressive surgical and/or early adjuvant therapy.(Arch Surg. 1995;130:585-589)

×