March 1997

Lymphatic Diameters and Radionuclide Clearance in a Murine Melanoma Model

Author Affiliations

From the Departments of Surgery (Drs Nathanson and Sarantou and Ms Anaya) and Radiation Oncology (Drs Avery and Hetzel), Case Western Reserve University at Henry Ford Health System, Detroit, Mich.

Arch Surg. 1997;132(3):311-315. doi:10.1001/archsurg.1997.01430270097020

Objectives:  To determine the clearance of a radionuclide from various sizes of footpad melanomas via lymphatics and to measure the diameters of these vessels.

Design:  Nonrandomized animal study.

Setting:  A hospital research laboratory.

Subjects:  C57BL/6 mice.

Interventions:  Female mice were injected in the right rear footpad with B16 F10 cells that were allowed to grow to either 1, 2, 3, 4, or 5 mm in anteroposterior diameter. Clearance from feet with or without tumors of injected technetium Tc 99m human serum albumin (99mTcHSA) was assessed for 200 minutes. Calf lymphatic diameters were measured using aniline blue dye.

Results:  The clearance of the injected99mTcHSA from mouse footpads without tumors was 1.26±0.18×10−4 mL/min×cm3 of tissue. Clearance increased 2.24-fold to 2.82±0.12×10−4 mL/min×cm3 of tissue from 1-mm tumors and to 6.20±0.08, 6.11±0.13, 6.91±0.58, and 7.23±0.48×10−4 mL/min×cm3 of tissue from 2-, 3-, 4-, and 5-mm tumors, respectively (P<.05). Calf lymphatic diameters increased from 75.41±9.72 μm in naive, nontumor-bearing mice to 93.51±7.12, 111.61±27.07, 126.69±25.20, 124.43±24.75, and 127.44±25.35 μm 1-, 2-, 3-, 4-, and 5-mm tumors, respectively (P<.01).

Conclusions:  There was a size-dependent, direct correlation between increasing tumor size in the footpad and increasing diameter of lymphatics draining the footpad. Clearance of injected99mTcHSA from these tumors also exhibited a similar positive correlation with tumor size.Arch Surg. 1997;132:311-315