[Skip to Content]
Sign In
Individual Sign In
Create an Account
Institutional Sign In
OpenAthens Shibboleth
[Skip to Content Landing]
February 1970

Experimental Technique for Perfusion of the Canine Donor Heart In Vitro

Author Affiliations

Loma Linda, Calif
From the Surgical Research Laboratory, Department of Surgery, Loma Linda University School of Medicine, Loma Linda, Calif.

Arch Surg. 1970;100(2):129-131. doi:10.1001/archsurg.1970.01340200017003

Modification of graft antigenicity poses an attractive alternative to the complex problems arising from host immunosuppression. Preliminary reports1-5 suggest that treatment of simple organs such as skin with soluble RNA prior to transplantation prolongs graft survival without the addition of immunosuppressive agents. In seeking to apply this principle to more complex organs such as the heart, it becomes necessary to develop some means for isolated organ support during this type of treatment. Techniques6-10 utilizing hypothermia or hyperbaric oxygenation or both are unsatisfactory for organ pretreatment. Previously described perfusion techniques, as summarized by Hardy et al11 are traumatic and hazardous to tissue preservation. It is the purpose of this communication to describe a simplified perfusion preparation developed and used in our laboratory for pretreatment and preservation of canine donor hearts for transplantation. This technique has proved to be rapid and inexpensive as well as atraumatic to the heart.


Groth, C.G., et al:  Effect of Ribonucleic Acid Perfusion on Canine Kidney and Liver Homograft Survival , Surgery 64:31-38 ( (July) ) 1968.
Jolley, W.B., and Hinshaw, D.B.:  Studies on Prolonged Homograft Survival , Amer J Surg 108:239-244 ( (Aug) ) 1964.Article
Jolley, W.B., and Hinshaw, D.B.:  Studies on Homograft Acceptance Including Early Clinical Results , Amer J Surg 112:308-313 ( (Aug) ) 1966.Article
Lemperle, G.; Michaels, W.; and Wieczorek, U.:  Autoradiographic Studies on the Incorporation of Foreign RNA and DNA Into Kidney Allografts , abstracted Europ Surg Res 1:219, 1969.Article
Seinfeld, H., and Brendel, W.:  Prolonged Function of Xenogenic Kidneys After Perfusion With an Unspecific RNA , abstracted Europ Surg Res 1:219-220, 1969.
Almond, C.H., et al:  Heart Preservation Utilizing Hyperbaric Oxygen and Hypothermia , Amer Heart J 69:425-426 ( (March) ) 1965.Article
Almond, C.H., et al:  Cardiac Preservation Utilizing Hyperbaric Oxygen and Hypothermia , Dis Chest 49:41-45 ( (Jan) ) 1966.Article
Block, J.H., et al:  Heart Preservation In Vitro With Hyperbaric Oxygenation and Hypothermia , J Thorac Cardiovasc Surg 48:969-983 ( (Dec) ) 1964.
Largiader, F., et al:  In Vitro Preservation of Canine Heart and Lung , Arch Surg 91:801-804 ( (Nov) ) 1965.Article
Lower, R.R., et al:  Successful Homotransplantation of the Canine Heart After Anoxic Preservation for Seven Hours , Amer J Surg 104:302-306 ( (Aug) ) 1962.Article
Hardy, J.D., et al:  Heart Transplantation in Infant Calves: Evaluation of Coronary Perfusion to Preserve Organ During Transfer , Ann NY Acad Sci 120:786-788 ( (Nov 30) ) 1964.Article
 Blood Gases Monitored Continuously During Intensive Care, Surgery , Medical News, JAMA 205:27 ( (Aug 5) ) 1968.
DeBurgh Daly, I., and Thorpe, W.V.:  An Isolated Mammalian Heart Preparation Capable of Performing Work for Prolonged Periods , J Physiol 79:199-217 ( (Sept) ) 1933.
Robicsek, F., et al:  The Maintenance of Function of the Donor Heart in the Extracorporeal Stage and During Transplantation , Ann Thorac Surg 6:330-342 ( (Oct) ) 1968.Article
Lower, R.R.; Stofer, R.C.; and Shumway, N.E.:  Homovital Transplantation of the Heart , J Thorac Cardiovasc Surg 41:196-204 ( (Feb) ) 1961.
Cooley, D.A., et al:  Transplantation of the Human Heart , JAMA 205:479-486 ( (Aug) 12) ) 1968.Article