[Skip to Content]
Sign In
Individual Sign In
Create an Account
Institutional Sign In
OpenAthens Shibboleth
[Skip to Content Landing]
February 1987

Inhibition of Lymphocyte Proliferation by Fluid-Phase C3b as Influenced by Macrophages

Author Affiliations

From the Departments of Surgery (Drs C. Ogle and Alexander) and Biological Chemistry (Dr J. Ogle), University of Cincinnati College of Medicine and Shriners Burns Institute (Mss Johnson and Keynton).

Arch Surg. 1987;122(2):234-238. doi:10.1001/archsurg.1987.01400140116016

• The effect of fluid-phase C3b on mitogen-induced lymphocyte proliferation in the presence and absence of macrophages was studied. In general, C3b inhibited the proliferation of lymphocytes when monocytes (macrophages) were present. The degree of inhibition by C3b was different for B and T lymphocytes and varied for different subpopulations of lymphocyte classes. In the absence of monocytes (macrophages), there was insignificant inhibition by C3b of lymphocyte proliferation, and thus the observed inhibition appeared to be due to the effect of C3b on the monocytes/macrophages present in the mixed lymphocyte preparations.

(Arch Surg 1987;122:234-238)

Tack BF:  The β Cys-γ Glu thiolester bond in human C3, C4 and α-2-macroglobulin . Springer Semin Immunopathol 1983;6:259-286.Article
Aranout MA, Melamed J, Tack BF, Colten HR:  Characterization of the human complement (C3b) receptor with a fluid phase C3b dimer . J Immunol 1981;127:1348-1354.
Fearon DT:  Identification of the membrane glycoprotein that is the C3b receptor of the human erthrocyte, polymorphonuclear leukocyte, B lymphocyte and monocyte . J Exp Med 1980;152:20-30.Article
Ogle JD, Ogle CK, Alexander JW:  Inhibition of neutrophil function by fluid phase C3b of complement . Infect Immun 1983;40:967-976.
Ogle CK, Ogle JD, Alexander JW:  Inhibition of bacterial clearance in the guinea pig by fluid phase C3b . Arch Surg 1984;119:57-60.Article
Alexander JW, Ogle CK, Stinnett JD, et al:  A sequential, prospective analysis of immunologic abnormalities and infection following severe thermal injury . Ann Surg 1978;188:809-816.Article
Fleisher TA, Berger M:  Immunoregulatory effects of C3 and its major cleavage fragments . Clin Immunol Immunopathol 1984;33:391-401.Article
Needleman BW, Weiler JW, Feldbush TL:  The third component of complement inhibits human lymphocyte blastogenesis . J Immunol 1981; 126:1586-1591.
Meuth JL, Morgan EL, Descipio RG, et al:  Suppression of T lymphocyte functions by human C3 fragments: Distribution of human T cell proliferative responses by a kallikrein cleavage fragment of human C3bi . J Immunol 1983;130:2605-2611.
Tsokos GC, Berger M, Balow JE:  Modulation of human B cell immunoglobulin reaction by the C3b component of complement . J Immunol 1984;132:622-627.
Weiler JM, Ballas ZK, Feldkush TL, et al:  Inhibition of human lymphocyte blastogenesis by C3: The role of serum in the tissue culture medium . Immunology 1982;45:247-252.
Goodwin JS, Bankhurst AD, Messner RP:  Suppression of human T cell mitogenesis by prostaglandin: Existence of a prostaglandin-producing suppressor cell . J Exp Med 1977;146:1719-1734.Article
Allison AC:  Mechanisms by which activated macrophages inhibit lymphocyte responses . Immunol Rev 1978;40:3-27.Article
Mauer U, Burger R, Steldern DV, et al:  Expression of Ia antigens on macrophages is reduced after stimulation with homologous C3b . J Immunol 1984;132:2802-2805.
Krakauer T:  A macrophage-derived factor that inhibits the production and action of interleukin 2 . J Leukocyte Biol 1985;38:429-439.
Nydegger UE, Zubler RH, Gabay R, et al:  Circulating complement breakdown products in patients with rheumatoid arthritis . J Clin Invest 1976;59:862-867.Article