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April 1987

Monoclonal Antibody—Mediated Modulation of Parathyroid Hormone Secretion by Dispersed Parathyroid Cells

Author Affiliations

From the Departments of Medicine (Drs Posillico and Brown) and Surgery (Dr Wilson), Brigham and Women's Hospital and Harvard Medical School, Boston; Department of Medicine, Joslin Diabetes Center and Harvard Medical School, Boston (Drs Srikanta and Eisenbarth); Department of Immunology, The Hospital for Sick Children, Toronto (Drs Letarte and Quackenbush); and Department of Immunology, Scripps Clinic and Research Foundation, La Jolla, Calif (Drs Quaranta and Kajaji).

Arch Surg. 1987;122(4):436-442. doi:10.1001/archsurg.1987.01400160062009

• Available data suggest that ionized calcium may interact with a cell surface "sensor" or "receptor" to produce changes in one or more intracellular second messengers that ultimately regulate the release of parathyroid hormone (PTH). Recently, we developed a series of monoclonal antibodies directed toward specialized differentiation antigens expressed on endocrine cells. Since many of these monoclonal antibodies displayed exquisite specificity for cell surface molecules on the parathyroid cell, we used these reagents as probes to investigate signal recognition/transduction mechanisms associated with abnormal calcium-regulated PTH secretion. Depending on their binding site on the respective target antigen molecules, these monoclonal antibodies either stimulated or inhibited hormone secretion. Thus, defects in membraneassociated structures may contribute to deranged calciumregulated PTH secretion in abnormal parathyroid cells.

(Arch Surg 1987;122:436-442)

Pearse AGE, Takor TT:  Neuroendocrine embryology and the APUD concept . Clin Endocrinol 1976;5( (suppl) ):229-244.Article
Satoh J, Prabhakar BS, Haspel MV, et al:  Human monoclonal autoantibodies that react with multiple endocrine organs . N Engl J Med 1983;309: 217-220.Article
Eisenbarth GS, Jackson RA, Srikanta S, et al:  Organ specific autoimmunity: T cells and target cells probed with monoclonal antibodies , in Forti G, Serio M, Lipsett MB (eds): Monoclonal Antibodies: Basic Principles, Experimental and Clinical Applications in Endocrinology , Serono Symposia Publications. New York, Raven Press, 1986, vol 30, pp 15-28.
Srikanta SS, Eisenbarth GS:  Islet cell antigens: Initial studies of their biology and function . Mol Biol Med 1986;3:113-127.
Haynes BF, Hemler ME, Mann DL, et al:  Characterization of a monoclonal antibody (4F2) that binds to human monocytes and to a subset of activated lymphocytes . J Immunol 1981;126:1409-1414.
Quackenbush EJ, Letarte M:  Identification of several cell surface proteins of non-T, non-B acute lymphoblastic leukemia by using monoclonal antibodies . J Immunol 1985;134:1276-1285.
Brown EM, Hurwitz S, Aurbach GD:  Preparation of viable isolated bovine parathyroid cells . Endocrinology 1976;99:1582-1586.Article
Brown EM, Leombruno R, Thatcher J, et al:  The acute secretory response to alterations in extracellular calcium concentration and dopamine in perifused bovine parathyroid cells . Endocrinology 1985;116:1123-1131.Article
Shoback D, Thatcher J, Leombruno R, et al:  Effects of extracellular Ca+ + and Mg+ + on cytosolic Ca+ + and PTH release in dispersed bovine parathyroid cells . Endocrinology 1983;113:424-429.Article
Tsien RY, Pozzan T, Rink TJ:  Calcium homeostasis in intact lymphocytes: Cytoplasmic free calcium monitored with a new intracellular-trapped fluorescent indicator . J Cell Biol 1982;94:325-335.Article
Posillico JT, Srikanta SS, Eisenbarth GS, et al:  Binding of monoclonal antibody (4F2) to its cell surface antigen on dispersed adenomatous parathyroid cells raises cytosolic calcium and inhibits parathyroid hormone secretion . J Clin Endocrinol 1987;64:43-50.Article
Posillico JT, Srikanta SS, Eisenbarth GS, et al:  Modulation of parathyroid cell secretory function by anti—islet cell surface monoclonal antibodies , abstracted. Clin Res 1985;33:285.
Michalak M, Quackenbush EJ, Letarte M:  Inhibition of Na+/Ca++ exchanger activity in cardiac and skeletal muscle sarcolemmal vesicles by monoclonal antibody 44D7 . J Biol Chem 1986;261:92-95.
Quackenbush EJ, Linsley P, Letarte M:  Mouse L cells express a molecular complex carrying the human epitopes recognized by monoclonal antibodies 44D7 and 44H7 after DNA-mediated gene transfer . J Immunol 1986;137:234-239.
Bottazo GF, Florin-Christensen A, Doniach D:  Islet cell antibodies in diabetes mellitus with autoimmune polyendocrine deficiencies . Lancet 1974;2:1279-1285.Article
Posillico JT, Wortsman J, Srikanta SS, et al:  Parathyroid cell surface autoantibodies that inhibit parathyroid hormone secretion from dispersed human parathyroid cells . J Bone Mineral Res 1986;1:475-483.Article
Maclaren N, Neufeld ND:  Autoimmunity and endocrine diseases , in Collee R (ed): Pediatric Endocrinology . New York, Raven Press, 1981, pp 597-631.
Hemler ME, Strominger JL:  Characterization of the antigen recognized by monoclonal antibody (4F2): Different molecular forms on human T and B lymphoblastoid cell lines . J Immunol 1982;129:623-630.
Posillico JT, Brown EM, Srikanta SS, et al: Role of the 4F2 cell surface antigen in the modulation of cytosolic calcium: Studies with mouse L cells that express the gene encoding for the human 4F2 antigen, abstract 24. Programs and abstracts of the Ninth International Conference on Calcium Regulating Hormones, Nice, France, 1986.