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January 1990

Superoxide Production by Wound NeutrophilsEvidence for Increased Activity of the NADPH Oxidase

Author Affiliations

From the Department of Surgery, School of Medicine, University of California, San Francisco.

Arch Surg. 1990;125(1):65-69. doi:10.1001/archsurg.1990.01410130071009

• Oxygen radical secretion by neutrophils is potentiated or "primed" by extravascular migration into wounds. To define this change in responsiveness more precisely we measured superoxide production by blood and wound neutrophils from rabbits using formylmethionyl-leucyl-phenylalanine and phorbol myristate acetate as agonists. In all experiments, the time- and dose-dependency of superoxide secretion were the same for blood and wound neutrophils. However, wound neutrophils produced significantly more superoxide. Furthermore, the cytochrome b component of the NADPH oxidase was found in greater quantities within wound neutrophils. We conclude that priming does little to alter the requirements for activating the NADPH oxidase but does significantly increase the velocity of superoxide generation. The data suggest that alterations in the assembly and function of the NADPH oxidase may contribute to enhanced superoxide secretion by wound neutrophils.

(Arch Surg. 1990;125:65-69)

Thomsen P, Bjursten LM, Ericson LE.  Joint fluid leukocyte activation by preformed immune complexes . Inflammation . 1986;10:243-256.Article
Holman RG, Maier RV.  Superoxide production by neutrophils in a model of adult respiratory distress syndrome . Arch Surg . 1988;123: 1491-1495.Article
Zimmerli W, Bruce Seligmann, Gallin JI.  Exudation primes human and guinea pigs neutrophils for subsequent responsiveness to the chemotactic peptide N-formylmethionylleucylphenylalanine and increases complement component C3bi receptor expression . J Clin Invest . 1986;77:925-932.Article
Paty PB, Graeff RW, Waldman FM, Hunt TK, Mathes SJ.  Biologic priming of neutrophils in subcutaneous wounds . Arch Surg . 1988;123:1509-1513.Article
McPhail LC, Clayton CC, Snyderman R.  The NADPH oxidase of human polymorphonuclear leukocytes: evidence for regulation by multiple signals . J Biol Chem . 1984;259:5768-5775.
GayJC, Beckman JK, Brash AR, OatesJA, Lukens JN.  Enhancement of chemotactic factor-stimulated neutrophil oxidative metabolism by leukotriene B4 . Blood . 1984;64:780-785.
Berkow RL, Wang D, Larrick JW, Dodson RW, Howard TH.  Enhancement of neutrophil superoxide production by preincubation with recombinant human tumor necrosis factor . J Immunol . 1987;139:3783-3791.
Danner RL, Joiner KA, Parillo JE.  Inhibition of endotoxin-induced priming of human neutrophils by lipid X and 3-aza-lipid x . J Clin Invest . 1987;80:605-612.Article
Metcalf JA, Gallin JI, Nauseef WM, Root RK.  Functions related to microbicidal activity . In: Laboratory Manual of Neutrophil Function . New York, NY: Raven Press; 1986:126-128.
Metcalf JA, Gallin JI, Nauseef WM, Root RK.  Intracellular granule constituents . In: Laboratory Manual of Neutrophil Function . New York, NY: Raven Press; 1986:149-150.
Baggiolini M, Hirsch JG, de Duve C.  Resolution of granules from rabbit heterophil leukocytes into distinct populations by zonal sedimentation . J Cell Bio.l 1969;40:529-541.Article
Baggiolini M, Hirsch JG, de Duve C.  Further biochemical and morphological studies of granule fractions from rabbit heterophil leukocytes . J Cell Biol . 1970;45:586-597.Article
Charon JA, Metzger Z, Hoffeld JT, Oliver C, Gallin JI, Mergenhagen SE.  An in vitro study of neutrophils obtained from the normal gingival sulcus . J Periodont Res . 1982;17:614-625.Article
Turner RA, Schumacher HR, Myers AR.  Phagocytic function of polymorphonuclear leukocytes in rheumatic diseases . J Clin Invest . 1973;52:1632-1635.Article
Zimmerli W, Lew PD, Cohen HJ, Waldvogel FA.  Comparative superoxide-generating system of granulocytes from blood and peritoneal exudates . Infect Immun . 1984;46:625-630.
Sadler KL, Badwey JA.  Second messengers involved in superoxide production by neutrophils: function and metabolism . Hematol Oncol Clin North Am . 1988;2:185-199.
Bass DA, Gerald C, Olbrantz P, Wilson J, McCall CE, McPhail LC.  Priming of the respiratory burst of neutrophils by diacylglycerol: independence from activation or translocation of protein kinase C. J Biol Chem . 1987;262:6643-6649.
Garcia RC, Segal AW.  Phosphorylation of the subunits of cytochrome b−245 upon triggering of the respiratory burst of human neutrophils and macrophages . Biochem J . 1988;252:901-904.
Segal AW.  Absence of both cytochrome b−245 subunits from neutrophils in X-linked chronic granulomatous disease . Nature . 1987;326:88-91.Article
Segal AW, Cross AR, Garcia RC, et al.  Absence of cytochrome b−245 in chronic granulomatous disease . N Engl J Med . 1983;308:245-251.Article
Borregaard N, Heiple JM, Simons ER, Clark RA.  Subcellular localization of the b-cytochrome component of the human neutrophil microbicidal oxidase: translocation during activation . J Cell Biol . 1983;97:52-61.Article