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Article
June 1992

Papillomavirus Found in Anorectal Squamous Carcinoma, Not in Colon Adenocarcinoma

Author Affiliations

From the Departments of Pathology (Drs Shroyer and Franklin) and Surgery (Drs Kim and Pearlman), University of Colorado Health Sciences Center, Denver; and the Department of Infectious Diseases (Dr Manos and Ms Greer), Cetus Corporation, Emeryville, Calif.

Arch Surg. 1992;127(6):741-744. doi:10.1001/archsurg.1992.01420060121018
Abstract

• We used polymerase chain reaction DNA amplification methods for the detection and typing of genital human papillomaviruses in paraffin-embedded tissue sections of five patients with anorectal squamous cell carcinoma and 22 patients with colonic adenocarcinoma. The cases were further tested by in situ hybridization with biotin-labeled probes specific for human papillomavirus types 6/11, 16/18, and 31/33/35. By polymerase chain reaction, human papillomavirus DNA was demonstrated in all of the cases of anorectal squamous cell carcinoma and in none of the cases of colonic adenocarcinoma for which analyzable DNA was available. Tumor cell nuclei stained for human papillomavirus DNA by in situ hybridization in four of the five cases of squamous cell carcinoma and in none of the cases of coIonic adenocarcinoma. We conclude that human papillomavirus types usually associated with malignant transformation are uniformly present in anorectal squamous cell carcinoma but are absent from adenocarcinoma of the colon.

(Arch Surg. 1992;127:741-744)

References
1.
Richart RM.  Causes and management of cervical intraepithelial neoplasia . Cancer . 1987;60:1951-1959.Article
2.
Barnes W, Delgado G, Kurman RJ, et al.  Possible prognostic significance of human papillomavirus type in cervical cancer . Gynecol Oncol . 1988;29:267-273.Article
3.
Gal AA, Meyer PR, Taylor CR.  Papillomavirus antigens in anorectal condyloma and carcinoma in homosexual men . JAMA . 1987;257:337-340.Article
4.
Palmer JG, Shepherd NA, Jass JR, Crawford LV, Northover JMA.  Human papillomavirus type 16 DNA in anal squamous cell carcinoma . Lancet . 1987;2:42.Article
5.
Palmer JG, Scholefield JH, Coates PJ, et al.  Anal cancer and human papillomaviruses . Dis Colon Rectum . 1989;32:1016-1022.Article
6.
Scholefield JH, Mclntyre P, Palmer JG, Coates PJ, Shepherd NA, Northover JMA.  DNA hybridization of routinely processed tissue for detecting HPV DNA in anal squamous cell carcinomas aver 40 years . J Clin Pathol . 1990;43:133-136.Article
7.
Wells M, Griffiths S, Lewis F, Bird CC.  Demonstration of human papillomavirus types in paraffin processed tissue from human anogenital lesions by in situ DNA hybridization . J Pathol . 1987;152:77-82.Article
8.
Gal AA, Saul SH, Stoler MH.  In situ hybridization analysis of human papillomavirus in anal squamous cell carcinoma . Mod Pathol . 1989;2:439-443.
9.
Wolber R, Dupuis B, Thiyagaratnam P, Owen D.  Anal cloacogenic and squamous carcinomas: comparative histologic analysis using in situ hybridization for human papillomavirus DNA . Am J Surg Pathol . 1990;14:176-182.Article
10.
Beckman AM, Daling JR, Sherman KJ, et al.  Human papillomavirus infection and anal cancer . Int J Cancer . 1989;43:1042-1049.Article
11.
Palefsky JM, Holly EA, Gonzales J, Berline J, Ahn DK, Greenspan JS.  Detection of human papillomavirus DNA in anal intraepithelial neoplasia and anal cancer . Cancer Res . 1991;51:1014-1019.
12.
Kiyabu MT, Shibata D, Arnheim N, Martin WJ, Fitzgibbons PL.  Detection of human papillomavirus in formalin-fixed, invasive squamous carcinomas using the polymerase chain reaction . Am J Surg Pathol . 1989;13:221-224.Article
13.
Wilczynski SP, Walker J, Liao SY, Bergen S, Berman M.  Adenocarcinoma of the cervix associated with human papillomavirus . Cancer . 1988;62:1331-1336.Article
14.
Nielsen AL.  Human papillomavirus type 16/18 in uterine cervical adenocarcinoma in situ and adenocarcinoma . Cancer . 1990;65:2588-2593.Article
15.
Kirgan D, Manalo P, McGregor B.  Immunohistochemical demonstration of human papilloma virus antigen in human colon neoplasms . J Surg Res . 1990;48:397-402.Article
16.
Kirgan D, Manalo P, Hall M, McGregor B.  Association of human papillomavirus and colon neoplasms . Arch Surg . 1990;125:862-865.Article
17.
Boguszakova L, Hirsch I, Brichacek B, et al.  Absence of cytomegalovirus, Epstein Barr virus, and papillomavirus DNA from adenoma and adenocarcinoma of the colon . Acta Virol . 1988;32:303-308.
18.
Manos MM, Ting Y, Wright DK, Lewis AJ, Broker TR, Wolinsky SM.  Use of polymerase chain reaction amplification for the detection of genital human papillomaviruses . In: Furth M, Greaves M, eds. Cancer Cells 7: Molecular Diagnostics of Human Cancer . New York, NY: Cold Spring Harbor Laboratory Press; 1989:209-214.
19.
Bauer HM, Ting Y, Greer CE, et al.  Genital human papillomavirus infection in female university students as determined by a PCR-based method . JAMA . 1991;265:472-477.Article
20.
Pascal RR, Perzin KH, Fenoglio-Preiser CM.  Neoplastic diseases of the small and large intestine . In: Silverberg SG, ed. Principles and Practice of Surgical Pathology . 2nd ed. New York, NY: Churchill Livingstone Inc; 1990:1185-1239.
21.
Wright DK, Manos MM.  Sample preparation from paraffinembedded tissues . In: Innis MA, Gelfand DH, Sninsky JJ, White JJ, eds. PCR Protocols: A Guide to Methods and Applications . Orlando, Fla: Academic Press Inc; 1990:153-158.
22.
Resnick RM, Correlissen MTE, Write DK, et al.  Detection and typing of HPV in archival cervical cancer specimens using DNA amplification with consensus primers . J Natl Cancer Inst . 1990;82:1477-1484.Article
23.
Nakajima-lijima S, Hamada H, Reddy P, Kakunaga T.  Molecular structure of the human cytoplasmic β-actin gene: interspecies homology of sequences in the introns . Proc Natl Acad Sci USA . 1985;82:6133-6137.Article
24.
Ting Y, Manos MM.  Detection and typing of genital human papillomaviruses . In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR Protocols: A Guide to Methods and Applications . Orlando, Fla: Academic Press Inc; 1990:356-367.
25.
Nuovo G.  Evidence against a role for human papillomavirus in colon neoplasms . Arch Surg . 1991;126:656.Article
26.
Syrjänen KJ.  Biology of human papillomavirus (HPV) infections and their role in squamous cell carcinogenesis . Med Biol . 1987;65:21-39.
27.
Koulos J, Symmans F, Chumas J, Nuovo G.  Human papillomavirus detection in adenocarcinoma of the anus . Mod Pathol . 1991;4:58-61.
28.
Daling JR, Weiss NS, Klopfenstein LL, Cochran LE, Chow WH, Daifuku R.  Correlates of homosexual behavior and the incidence of anal cancer . JAMA . 1982;247:1988-1990.Article
29.
Frazer IH, Crapper RM, Medley G, Brown TC, Mackay IR.  Association between anorectal dysplasia, human papillomavirus, and human immunodeficiency virus infection in homosexual men . Lancet . 1986;2:657-660.Article
30.
Nash G, Allen W, Nash S.  Atypical lesions of the anal mucosa in homosexual men . JAMA . 1986;256:873-876.Article
31.
Palefsky JM, Gonzales J, Greenblatt RM, Ahn DK, Hollander H.  Anal intraepithelial neoplasia and anal papillomavirus infection among homosexual males with group IV HIV disease . JAMA . 1990;263:2911-2916.Article
32.
Kiviat N, Rompalo A, Bowden R, et al.  Anal human papillomavirus infection among human immunodeficiency virus-seropositive and seronegative men . J Infect Dis . 1990;162:358-361.Article
33.
Kirgan D, Manalo P, Hall M, McGregor B.  Association of human papillomavirus and colon neoplasms , Arch Surg . 1990;125:862-865.Article
34.
Cheng J-Y, Meng C-L, Chao C-F, Gau S-D, Lin J-C.  Human papillomavirus type-related DNA and c-myc oncogene alterations in colon cancer cell lines . Dis Colon Rectum . 1991;34:469-474.Article
35.
Manalo P, Highison G, McGregor B, Hall M.  Electronmicroscopic evidence of papillomavirus in human colon neoplasms . Am J Clin Pathol . 1991;96:413.
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