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Article
October 1992

Protective Effect of Anisodamine on Cultured Bovine Pulmonary Endothelial Cell Injury Induced by Oxygen-Free Radicals

Author Affiliations

From the Department of Pathophysiology, Hunan Medical University, Changsha, People's Republic of China.

Arch Surg. 1992;127(10):1204-1209. doi:10.1001/archsurg.1992.01420100062010
Abstract

• Anisodamine, a Chinese traditional medicine herb, has been used for treatment of adult respiratory distress syndrome effectively, but little is known about its mechanism. We attempted to investigate if anisodamine could protect bovine pulmonary endothelial cell injury induced by exogenous oxygen-free radicals that were generated by xanthine/xanthine oxidase or opsonized zymosan—stimulated polymorphonuclear leukocytes. Results showed that with the addition of xanthine/xanthine oxidase into cultured bovine pulmonary endothelial cells, production of malondialdehyde and release of lactate dehydrogenase in supernatant increased, and synthesis of prostacyclin decreased. Damaged cellular membranes were revealed by scanning electron microscopy. The same was true for the addition of opsonized zymosan—stimulated polymorphonuclear leukocytes. While treatment with anisodamine greatly attenuated all of the above-mentioned parameters, results showed that (1) cultured bovine pulmonary endothelial cells could be damaged by oxygen-free radicals, (2) anisodamine had a protective effect on this injury as effective as that of superoxide dismutase and catalase, and (3) the membrane-stable action might contribute to the mechanism of protective effect against this injury.

(Arch Surg. 1992;127:1204-1209)

References
1.
Ge M, Luo Z-Y, Luo H, You J-L.  Effect of anisodamine on pulmonary function of disaggregating platelet microaggregates in canine endotoxemia . Bull Hunan Med Coll . 1985;10:7-12.
2.
Su J-Y, Hock CE, Lefer AM.  Beneficial effect of anisodamine in hemorrhagic shock . Pharmacologist . 1983;25:265.
3.
Hock CE, Su J-Y, Lefer AM.  Salutary effect of anisodamine in murine traumatic shock . Circ Shock . 1983;11:211-216.
4.
Luo Z-Y, Xu L, Luo H.  Effect of anisodamine on glucocorticoid receptor of pulmonary alveolar macrophage in rabbits with oleic acid—induced respiratory distress syndrome . Circ Shock . 1987;21:349. Abstract.
5.
Yu X-Y, Luo Z-Y, You J-L, Luo H.  The influence of anisodamine on the cells from bronchoalveolar lavage in experimental respiratory distress syndrome . Bull Hunan Med Coll . 1985;10:13-18.
6.
You J-L, Lu Y-Z, Wang Y-R, Luo Z-Y.  Therapeutic effects on arrhythmias induced by experimental myocardial reperfusion in rats . Bull Hunan Med Coll . 1986;11:11-14.
7.
Ryan US, Clements E, Habliston D, Ryan JW.  Isolation and culture of pulmonary artery endothelial cells . Tissue Cell . 1978;10:535-554.
8.
Sacks T, Moldow CF, Craddock PK, Bowers TK, Jacob HS.  Oxygenradicals mediate endothelial cell damage by complement-stimulated granulocytes . J Clin Invest . 1978;61:1161-1167.Article
9.
Goldstein IM, Rood D, Kaplan HB, Weissmann G.  Complement and immunoglobulins stimulate superoxide production by human leukocytes . J Clin Invest . 1975;56:1155-1163.Article
10.
Hjorth R, Jonsson AK, Verbland P.  A rapid method for purification of human granulocytes using Percoll . J Immunol Methods . 1981;43:95-101.Article
11.
Ohkawa H, Ohishi N, Yagi K.  Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction . Anal Biochem . 1979;95:351-358.Article
12.
Block R, Patal JM, Sheridan NP.  Effect of oxygen and endotoxin in lactatedehydrogenase release, 5-hydroxytryptamine uptake, and antioxidant enzyme activities in endothelial cells . J Cell Physiol . 1985;122:240-248.Article
13.
Whorton ME, Montogomery E, Kent RS.  Effect of hydrogen peroxide on prostaglandin production and cellular integrity in cultured porcine aortic endothelial cells . J Clin Invest . 1985;76:295-302.Article
14.
Jaffe EA.  Culture and identification of large-vessel endothelial cells . In: Jaffe EA, ed. Biology of Endothelial Cells . Dordrecht, the Netherlands: Martinus Nijhoff Publishers; 1984.
15.
Cochrand CC, Sprang R, Revak SD.  Pathogenesis of the adult respiratory distress syndrome: evidence of oxidant activity in bronchoalveolar lavage fluid . J Clin Invest . 1983;71:754.Article
16.
Hoidal JR, Fox BB, Marbe LV, Peri R, Repine JE.  Altered oxidative metabolic response in vitro of alveolar macrophages from asymptomatic cigarette smokers . Am Rev Respir Dis . 1981;123:85-89.
17.
Yasaka T, Ohya I, Mutsunoto T, Shiramiza T, Sasagugi Y.  Acceleration of lipid peroxidation in human parquet poisoning . Arch Intern Med . 1981; 141:1169-1171.Article
18.
Jackson RM, Veal CF, Alexander CB, Brannen AL, Fulmer JD.  Reexpansion pulmonary edema . Am Rev Respir Dis . 1988;137:1165-1171.Article
19.
Ratych SL, Chuknyisk RS, Bulkley GB.  The primary localization of free radical generation after anoxia/reoxygenation in isolated endothelial cells . Surgery . 1987;102:122-131.
20.
Lunch HJ, Grown CM, Gallagher KP, Bolling SF, Deeb CM, Morganboth ML.  Xanthine oxidase inhibition attenuates ischemia-reperfusion lung injury . J Surg Res . 1988;44:538.Article
21.
Deng G-H, Luo Z-Y, Yin D, Luo H.  Protective effects of anisodamine on lung injury induced by oleic acid in rabbits . Bull Hunan Med Univ . 1989; 14:339-342.
22.
Zhou Q-M, Zhan Q-S, Luo Z-Y.  Oxygen-free radical and lung injury, III: effect of tungsten on experimental lung injury . Bull Hunan Med Univ . 1990;15:131-135.
23.
Song D-K, Luo Z-Y.  The role of oxygen-free radicals in the pathogenesis of oleic acid—induced isolated rat lung injury and protective effect of anisodamine . Bull Hunan Med Univ . 1990;15:19-23.
24.
Xiu R-J, Hammerschmidt DE, Patricia PA, Harrys MS, Jacob HS.  Anisodamine inhibits thromboxane synthesis, granulocyte and platelet aggregation . JAMA . 1982;247:1458-1460.Article
25.
J-Y, Wu L, Song Z.  Comparison of antishock effect and lysosomal stabilization action of anisodamine and dexamethasone . Circ Shock . 1987; 21:322. Abstract.
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