http://pubs.jamanetwork.com/ en-us Fri, 01 Feb 2013 00:00:00 GMT Thu, 28 Feb 2013 21:48:31 GMT Silverchair editor@pubs.jamanetwork.com webmaster@pubs.jamanetwork.com http://pubs.jamanetwork.com/article.aspx?articleID=1655067 Fri, 01 Feb 2013 00:00:00 GMT Chernichenko N, Linkov G, Li P, et al. <span class="paragraphSection"><div class="boxTitle">Objective</div>To examine the therapeutic effects of an attenuated, replication-competent vaccinia virus (GLV-1h68) against a panel of 5 human salivary gland carcinoma cell lines.<div class="boxTitle">Design</div>The susceptibility of 5 salivary gland carcinoma cell lines to infection and oncolysis by GLV-1h68 was assessed in vitro and in vivo.<div class="boxTitle">Results</div>All 5 cell lines were susceptible to viral infection, transgene expression, and cytotoxic reactions. Three cell lines were exquisitely sensitive to infection by very low doses of GLV-1h68. Orthotopic parotid tumors exhibited more aggressive behavior compared with flank tumors. A single intratumoral injection of GLV-1h68 induced significant tumor regression without observed toxic effects in flank and parotid tumor models; controls demonstrated rapid tumor progression.<div class="boxTitle">Conclusion</div>These promising results demonstrate significant oncolytic activity by an attenuated vaccinia virus for infecting and lysing salivary gland carcinomas, supporting future clinical trials.</span> 139 2 173 182 10.1001/jamaoto.2013.1360 http://pubs.jamanetwork.com/article.aspx?articleID=1655067