In the routine examination of sections from the brain of a case of epidemic encephalitis, minute granules were found in degenerated nerve cells in the areas involved by the disease process. Although similar granules in many diseases of the central nervous system have been described in German publications, notably by Alzheimer1 and Achucarro,2 to my knowledge their presence in cases of epidemic encephalitis has not previously been noted, and it therefore seems worth while to describe them as part of the microscopic pathology of this disease.
The granules are best demonstrated in tissues fixed in Zenker's or Helly's fluid, embedded in paraffin and stained with eosin-methylene blue or by Bensley's modification of the Altmann stain (acid-fuchsinmethyl-green). To bring them out with eosin we used a combination of equal parts of strong alcoholic and 5 per cent. aqueous eosin, staining forty minutes at 56 C. This stain, made
MELENEY HE. DEGENERATION GRANULES IN BRAIN CELLS IN EPIDEMIC ENCEPHALITIS. Arch NeurPsych. 1921;5(2):146–149. doi:10.1001/archneurpsyc.1921.02180260035004
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