In studies of the so-called "silver staining methods," emphasis has usually been laid on the rôle of the fixative and on the nature of the silver solution, while relatively little attention has been paid to the action of the reducing agent. Perhaps the first investigator to make use of wide variations in this step was del Rio-Hortega. In the gradual development of his method of staining microglia, he parted from the traditional reduction with 10 or 20 per cent commercial formaldehyde solution and found that selective staining of the cells could be secured by immersing the silver-impregnated sections, without washing, directly into a formaldehyde solution of only 1 per cent (1 cc. of concentrated liquor formaldehydi—approximately 37 per cent—in 100 cc. of distilled water). He also made certain interesting observations on reduction after a variety of intervening washes with, for example, alcohol, pyridine and other liquids. These observations, which I
KUBIE LS. STAINING OF TISSUES OF THE CENTRAL NERVOUS SYSTEM WITH SILVERTHE INFLUENCE OF THE STRENGTH OF THE REDUCING AGENT. Arch NeurPsych. 1929;22(1):135–138. doi:10.1001/archneurpsyc.1929.02220010138012
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