The ideal technic for staining nerve tissue would allow one to stain by specific methods all the different structures in serial sections from one and the same block of embedded tissue. A step forward toward the accomplishment of this goal has been the method devised by Stern1 for the staining of microglia and oligodendroglia in celloidin sections. As indicated by his photomicrographs, the method gives excellent results, especially in cases of pathologic glial proliferation. We were able to confirm his results by following exactly his directions.
Even trained technicians, however, do not always succeed in the preparation of the silver solution devised by Stern, and, besides, the use of a 10 per cent commercial formaldehyde solution in connection with 10 per cent ammoniacal silver nitrate frequently leads to the production of disturbing silver precipitates (Kubie).2 Therefore, we have modified the method somewhat in order to make it useful