The difficulty of obtaining fresh, properly fixed tissue has unfortunately limited the usefulness of the gold and silver impregnation methods of Ramón y Cajal1 and del Rio-Hortega2 for demonstration of the neuroglia cells, particularly in the study of old formaldehyde-fixed material and of brains hardened intact in formaldehyde before sectioning. The deformalization and bromination proposed by Globus3 have given only partly satisfactory results in our hands.
The necessity for using frozen sections with most previously employed metallic staining methods has also been a distinct disadvantage at times. With many friable neoplastic tissues the difficulty of cutting satisfactorily thin and uniform sections on the freezing microtome and carrying them through many different solutions off the slides has diminished the value of the methods.
We have previously, on the advice of Bailey,4 employed Perdrau's5 method for silver impregnation of reticulin for paraffin sections, but this is the
Pilcher C, Owings E. A STAIN FOR NEUROGLIA IN FORMALDEHYDE-FIXED PARAFFIN SECTIONS. Arch NeurPsych. 1936;35(2):351–356. doi:10.1001/archneurpsyc.1936.02260020145011
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