The need for impregnating microglia in tissue fixed in a solution of formaldehyde has led to several modifications of Hortega's original methods. I present here a further modification that has the advantages of ease, rapidity and uniformly good results.
Cut frozen sections of material fixed in a solution of formaldehyde at from 20 to 25 microns, receiving them in tap water to which has been added about 20 drops of ammonia per hundred cubic centimeters.
Transfer sections directly to a 5 per cent solution of ammonium bromide and heat in the paraffin oven at from 45 to 50 C. for from ten to fifteen minutes.
Transfer directly to Hortega's mixture, of equal parts of ammonia, pyridine and water, for two minutes or longer.
Transfer directly to a freshly made solution of sodium sulfite, from 3 to 5 per cent, for two or three minutes.
Transfer directly to a solution
King LS. METHOD FOR RAPID IMPREGNATION OF MICROGLIA AND OLIGODENDROGLIA IN MATERIAL FIXED IN FORMALDEHYDE. Arch NeurPsych. 1937;38(2):362–364. doi:10.1001/archneurpsyc.1937.02260200134011
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