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November 1954

PATHOGENESIS OF HEPATOCEREBRAL DISEASE: II. Histochemical Study of Copper of Liver and Brain in Wilson's Disease

Author Affiliations


From the Third Internal Clinic of Prof. S. Okinaka, University of Tokyo School of Medicine.

AMA Arch NeurPsych. 1954;72(5):573-578. doi:10.1001/archneurpsyc.1954.02330050043007

IN OUR previous paper, on "a case of hepatolenticular degeneration,"1 we reported that we could confirm the definite increase of copper excretion in the urine and could demonstrate that dimercaprol (BAL) treatment gave rise to the marked increase of copper excretion. In this paper we report histochemical findings in the liver and brain in Wilson's disease (hepatolenticular degeneration). Recently, case reports of Wilson's disease are appearing rather frequently in Japan, and we were able to obtain five livers and two brains from autopsy materials of the School of Medicine of Tokyo, Tohoku Imperial University, Osaka University, and Saint Luke's Hospital of Tokyo. Copper granules were demonstrated histochemically in four of these five livers and in the putamen of one brain.

MATERIAL AND METHOD  Livers were obtained at autopsy and fixed in 10% formalin before examination. Histochemical demonstration of copper was performed by the method of Okamoto and Utamura,2

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