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June 1957

Cerebral Changes Revealed by Radioautography with S35-Labeled L-Methionine

AMA Arch NeurPsych. 1957;77(6):588-594. doi:10.1001/archneurpsyc.1957.02330360046003

This radioautographic study was undertaken to ascertain the fate of radiosulfur in the brains of rats receiving S35-labeled L-methionine under a variety of experimental conditions. As reported elsewhere,9,10 it was of great interest to find in the initial experiments that under normal conditions the cerebral radioautograms showed depths of contrast that gave them a striking resemblance to Nissl-stained sections. While this work was in progress, Cohn et al. published an abstract summarizing cerebral radioautographic studies in which they used the same labeled amino acid.3 By biochemical methods they found that the radioactivity was "mainly due to protein-bound S35." They observed that "the incorporation of S35 into the proteins was considerably greater in the regions containing cell bodies than in the white matter" and pointed out that "it was particularly prominent in the pyramidalis layer of Ammon's horn [hippocampus] and in the `neurosecretory cells' of